Sequences from confirmation amplicon


Confirmation sequences for line 782H09 and hit At5g39660 are shown.
Line ID 782H09
Locus At5g39660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15879251
Primers used for PCR TA28 - 8474, show primer details
   
Sequence Name 35-K030142-022-782-H09C-8409
GenBank Accession LN501707 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K030142-022-782-H09C-8409
TATCATAAAACTAAGATTTGACCNAATCTACATANACCCATNCATCATTTCCCATTTACA
NATCANAATCAATTCACAAGTTCCAAAAAAAACTAACCCTTTTTATCAGTCTCGCTCTCT
CCTCCACCATCACCAACATCATCACCTTCTTCTCGTCCTAAACCGGAATCACCCATCTCT
TCATCATCATCATCGCCGGTACACGAATCTGATAACCGAACAGGAATCTGAGTTTCGGTT
AAAATCCGGTATAGCTAGAAGAAAGAATCACNACACCAAGCTCAGGTAAAGGAATCGTCT
TTCAAAGAGCTTNATCGCCGGATCAGCCATGGTAATGATTCCTTTTCACTTCTTTCT
   
Sequence Name 35-K030142-022-782-H09C-TA28
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, TA28: 5'-TTTTTCTTTCTCTCTCGCAAAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K030142-022-782-H09C-TA28
TTTTCTCTGTCTCTCAAAGTTTCGCTTTTGCTCCTGGGAGAAGAAGAAGAGTGAAGGATC
TTACATGGTGATCGGCGATAGCTCTTGGAAGACATTCTTTACTGAGCTGGTGTTGTGATT
TCTCTCTACTTACGGATTTTACCGAACTCAGATCCTGTCGGTATCAGATCGTGTACGGCG
ATGATGATG

Last Updated on 06.12.2018 16:11