Sequences from confirmation amplicon


Confirmation sequences for line 783H06 and hit At5g04940 are shown.
Line ID 783H06
Locus At5g04940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1456030
Primers used for PCR HP50 - 8474, show primer details
   
Sequence Name 27-K033541-022-783-H06C-8409
GenBank Accession LN498279 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K033541-022-783-H06C-8409
ATTTACATTGAATAATCCTGCCCATACAGTAGATCCTGATTTCAATCAGGGCGTTTGATA
ATCCCACTAACTGCTTCCTTAGCATCCTCAAGTTGTGCAAACCTTCTTCTTAACGCATCA
AACCGCATAAGAACACTCAACACCAACTCCCTATTGCCATTCTCTCTATCAGCCACATTA
ATCCCACTCTCGAAATTCATATTCTCAGGCCGAGAAATGGGACGCTTTTTAGGGATCCTT
CTTTTCACAGTTGACCCCTCAAGTTCCGCGTTGCCATTAGACACATCAGGAGATCTAAAT
GACCTTAAAGGAGTAACCAACGAAGGCTCAGATGCATGCTGAGGAGGCTGCTGCTGATAT
ACCGGTGGTGGATTCTGAGGCTGCTGATGTTGCGGTGGATACTGAGCTTGGTTAAGATCT
GGTGTGTGCTGATTCGCTTGAGACGAACTAAACGGATAAAACGATGAGAACCCAGGAGGA
AATGGTCCAAAAGGAGGAGCACACACAAAAGGCGGAGCTTGATTTCCACTGGGAAACACA
GGTCTTAGATAGNCCAATGG
   
Sequence Name 27-K033541-022-783-H06C-HP50
GenBank Accession LN498280 [GenBank]
Sequencing primer gene specific primer, HP50: 5'-ACCATTGCGTACTCTAAGACCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K033541-022-783-H06C-HP50
TAGCTCGCCTTTTGTGTGTGCTCCTCCTTTTGGACCATTTCCTCCTGGGTTCTCATCGTT
TTATCCGTTTAGTTCGTCTCAAGCGAATCAGCACACACCAGATCTTAACCAAGCTCAGTA
TCCACCGCAACATCAGCAGCCTCAGAATCCACCACCGGTATATCAGCAGCAGCCTCCTCA
GCATGCATCTGAGCCTTCGTTGGTTACTCCTTTAAGGTCATTTAGATCTCCTGATGTGTC
TAATGGCAACGCGGAACTTGAGGGGTCAACTGTGAAAAGAAGGATCCCTAAAAAGCGTCC
CATTTCTCGGCCTGAGAATATGAATTTCGAGAGTGGGATTAATGTGGCTGATAGAGAGAA
TGGCAATAGGGAGTTGGTGTTGAGTGTTCTTATGCGGTTTGATGCGTTAAGAAGAAGGTT
TGCACAACTTGAGGATGCTAAGGAAGCAGTTAGTGGGATTATCAAACGCCCTGATTTGAA
ATCAGGATCTACTTGTATGGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11