Sequences from confirmation amplicon


Confirmation sequences for line 784D04 and hit At2g20960 are shown.
Line ID 784D04
Locus At2g20960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9007770
Primers used for PCR PG25 - 8474, show primer details
   
Sequence Name 46-K101745-0022-784-D04C-8409
GenBank Accession LN505817 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K101745-0022-784-D04C-8409
CGGAATGAGCCATTTACAATTGAATATATCCATGTAGATTTCCCGCACACCGCAACACCG
CTCTGCACACCAAGAGGACGCTTTTTCACTAGAAACTAGGCCGAGGACTCCTGAACGCCG
ATCCAATACCTCTGATACTAGGCCAAGGACACCCATACATGAGTCTGCGGCAACAGGAAG
AAGGCCTCAAACACCAGAAACTAGACCAAGGACTCCTGATCACCGATATGCCACCTATGA
TAATAGGCCGAGGACACCCATACATGAGTCTGCTGCAACAGAAAGGAGGCCCCAAACACC
AGAAACTAGGCCGAGGACTCCTGAACACCGATCTGCCATCCCTGACACTAGGCCGAGGAC
ACCTATACATGAGTCTGCTGCAACAGGAAGGAGGCCTCAAACACCAGAAACTAGGCCGAG
GACTGCACAACGTAGAGGGAGGTCACCTGAGTTCATGGAAAGATCCCCCGGACCAAGGTC
CAAGACCCCAGAGCCTCAACCTACTTACTTCGAACCATCCTCAAGGACTCCAAAACAGCG
GTCCAAGACGCCTGAACCCAGCCCTAGGATTCCCCAAACTCAGCCCATGTCCCACAGATC
CCTTGACAACGCTGCTCTTAAAATGCCTCGGACAGTTGAAACTAGGCCTAGGACGCCNGA
ACACCAGCCGAGGACTGTGGAAACAAAGCCNAGAATACATGANAGTAGGCCCAAAACTGC
AGTCTATGGAGGAAGGTCACCTGATCACAGGGAAAAAATCTCACAAGAAATGGGTCAAAG
ATCATCTCAGGCCTACNATTA
   
Sequence Name 46-K101751-0022-784-D04C-PG25
GenBank Accession LN505818 [GenBank]
Sequencing primer gene specific primer, PG25: 5'-GCTTTGCTCCCAAGATAATTGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K101751-0022-784-D04C-PG25
GACATTTCTTGTGAGATTTTTTCCCTGTGATCAGGTGACCTTCCTCCATAGACTGCAGTT
TTGGGCCTACTTTCATGTATTCTTGGCTTTGTTTCCACAGTCCTCGGCTGGTGTTCTGGC
GTCCTAGGCCTAGTTTCAACTGTCCGAGGCATTTTAAGAGCAGCGTTGTCAAGGGATCTG
TGGGACATGGGCTGAGTTTGGGGAATCCTAGGGCTGGGTTCAGGCGTCTTGGACCGCTGT
TTTGGAGTCCTTGAGGATGGTTCGAAGTAAGTAGGTTGAGGCTCTGGGGTCTTGGACCTT
GGTCCGGGGGATCTTTCCATGAACTCAGGTGACCTCCCTCTACGTTGTGCAGTCCTCGGC
CTAGTTTCTGGTGTTTGAGGCCTCCTTCCTGTTGCAGCAGACTCATGTATAGGTGTCCTC
GGCCTAGTGTCAGGGATGGCAGATCGGTGTTCAGGAGTCCTCGGCCTAGTTTCTGGTGTT
TGGGGCCTCCTTTCTGTTGCAGCAGACTCATGTATGGGTGTCCTCGGCCTATTATCATAG
GTGGCATATCGGTGATCAGGAGTCCTTGGTCTAGTTTCTGGTGTTTGAGGCCTTCTTCCT
GTTGCCGCAGACTCATGTATGGGTGTCCTTGGCCTAGTATCAGAGGTATTGGATCGGCGT
TCAGGAGTCCTCGGCCTAGTTTCTAGTGAAAAAGCGTCCTCTTGGTGTGCAGAGCGGTGT
TGCGGTGTGCGGGAAATCTACATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGGAGAAAAGAAAGAGTAATTAC
CCATTTTTTTTCATTTCAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11