Sequences from confirmation amplicon


Confirmation sequences for line 787D12 and hit At3g52760 are shown.
Line ID 787D12
Locus At3g52760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19554744
Primers used for PCR LB29 - 8474, show primer details
   
Sequence Name 68-K033720-022-787-D12C-8409
GenBank Accession LN511945 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>68-K033720-022-787-D12C-8409
GCCATTTACAATTGAATATAACTTCAAATTCGAGATCTGAATTAGGGACTCTAGAGGATG
ACGAAGGATTTTCCAATTCCACCGGTTGTTTTCCCCACCAGTGGATCATCTCCGGCCACC
GCAAATGTTCAGCAACGCCGAGTTCCCACAGCTCCTTACCAACCACCACGTCAATCCTCC
GCTTCATCTTCAATCCCTTTCATTCCATTTGAAATCGGATCCGCCGCCGTTTCAATGCCG
GCGGGACCATTCGACGGAACAATCGCTTCATCTGCATCTTTCGGTAGAGGAGTCTCTGCT
TCGTTTGAGGATGAGGAGCCTTTACTCGACGAACTCGGTATCCATCCAGATCAAATCTGG
AAGAAAACTAGATCGATTCTCAATCCTTTTCGAATCAATCAGACTGTTCACAAAGATTCA
GATCTCTCTGGTCCGATCTTCTTATACCTTGCGCTTTGCTTATTTCAATTGCTCGCTGGG
AAGATACAGTTTGGTGTTATACTTGGTTGGGTTGTTGTTTCTTCTATCTTCTTGTACATT
GTCTTCAATATGCTTGCTGGTAGAAATG
   
Sequence Name 30-K033720-022-787-D12C-LB29
GenBank Accession LN499621 [GenBank]
Sequencing primer gene specific primer, LB29: 5'-CATTTCTACCAGCAAGCATATTGA-3'
Sequence (A. th genome BLAST matches underlined)
>30-K033720-022-787-D12C-LB29
GAGATGAGAACAACACCCACCAAGTATAACACCAAACTGTATCTTCCCAGCGAGCAATTG
AAATAAGCAAAGCGCAAGGTATAAGAAGATCGGACCAGAGAGATCTGAATCTTTGTGAAC
AGTCTGATTGATTCGAAAAGGATTGAGAATCGATCTAGTTTTCTTCCAGATTTGATCTGG
ATGGATACCGAGTTCGTCGAGTAAAGGCTCCTCATCCTCAAACGAAGCAGAGACTCCTCT
ACCGAAAGATGCAGATGAAGCGATTGTTCCGTCGAATGGTCCCGCCGGCATTGAAACGGC
GGCGGATCCGATTTCAAATGGAATGAAAGGGATTGAAGATGAAGCGGAGGATTGACGTGG
TGGTTGGTAAGGAGCTGTGGGAACTCGGCGTTGCTGAACATTTGCGGTGGCCGGAGATGA
TCCACTGGTGGGGAAAACAACCGGTGGAATTGGAAAATCCTTCGTCATCCTCTAGAATCC
CTAATTCAGATCTCGAATTTGAAGTTATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCC
Primers used for PCR CT38 - 3144, show primer details
   
Sequence Name 20-K105032-0022-787-D12C-CT38
GenBank Accession LN495205 [GenBank]
Sequencing primer gene specific primer, CT38: 5'-AACATCAGAAAGGCAACTTTGAAT-3'
Sequence (A. th genome BLAST matches underlined)
>20-K105032-0022-787-D12C-CT38
TTTAACAATTCTAAATGACTTACGTATTGATCATTGCTCTTACAATTTATACAAATTCTA
AATGACTTTCAAAAAATGGTAAGGACACTACTATAGGTTTAAAAAATAAAAAATCGCCGT
TGCCGGGGATCGAACCCGGGTCACCCGCGTGACAGGCGGGAATACTTACCACTATACTAC
AACGACTTTGTTGTTCAGTGAGCCTAATAACTTATAAAATTACGATAATTAGTCAGTAGT
GAAAACTATTCACAACTCACAAGTGGGGCTATTGACTTGTTTGTGTAGCAAAGGCTGTTC
AAAACTACAAACTAATGGGCTGATAAAGCCTTATACGGCCCAAAACATTATTAATTCACT
TACCGAAAATGCCCTTATCACATATCACGAAGATACGAACACGAAGAATGCGTAGTAGTC
GCCTGTTACTGGAATTATTTTCAATTTCTGTTACAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAA
ATGGGGGCTTAGATGAGAAACTTCACGATCGATGCCTTGATTTCGCCATTCCCAGATACC
CATTTCATCTTCAGATTGGTCTGAGATTATGCGAAAATATACACTCATATACATAAATAC
TGACAGTTTGAGCTACCAATTCAGTGTAGCCCATTACCTTACATAATTCACTCAAATGCT
AGGCAGTCTGTCAACTCGGCGTCAATTTGTCGGCCACTATACGATAGTTGCGCAAATTTT
CAAAGTCCTGGCCTAACATCACACCTCTGTCGGCGGCGGGTCCCATTTGTGATAAAT
   
Sequence Name 36-K105033-0022-787-D12C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined)
>36-K105033-0022-787-D12C-3144
CTTGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCGGTTTAAACCCGAACACCGT
CACCATGCACTTTACTCTTCCACCATTGCTTGTAATGGAAGTAATGTCAGTGTTGACCTT
CTTAACTGGGAATCCAGTCATGGATTTGAGGCCGCCGAATGGAGCCACCGCGGCCGATTG
CACCGTAGAAGCACGGCTGACTGTAGTCACAGCTGAAGAGGATATCATAGAAGCCATTTT
TCTCACTTCTGTATGAATTGCAAAGCTGGTACGACGGATCGATCCACGTGTGGAAGATAT
GAATTTTTTTGAGAAACTAGATAAGATTAATGAATATCGGTGTTTTGGTTTTTTCTTGTG
GCCGTCTTTGTTTATATTGAGATTTTTCAAATCAGTGCGCAAGACGTGACGTAAGTATCC
GAGTCAGTTTTTATTTTTCTACTAATTTGGTCGTTTATTTCGGCGTGTAGGACATGGCAA
CCGGGCCTGAATTTCGCGGGTATTCTGTTTCTATTCCAACTTTTTCTTGATCCGCAGCCA
TTAACGACTTTTGAATAGATACGCTGACACGCCAAGCCTCGCTAGTCAAAAGTGTACCAA
ACAACGCTTTACAGCAAGAACGGAATGCGCGTGACGCTCGCGGTGACGCCATTTCGCCTT
TTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTA
CACTAGCATCTGAATTTCATAACCAATCTCGATACACCAAATCGGATCTTATAATTAAAT
GGCCTTCGCTGCCCATATTATTGGTAAACTCACAGCATCAATCACGGGATTTTTCTCGAA
TTAATTGCGTCGAATCTCAGCATCGAAATATTCGCCTTTTTCGTCCATTAGACTATCTAT
T

Last Updated on 06.12.2018 16:11