Sequences from confirmation amplicon


Confirmation sequences for line 788F09 and hit At2g25900 are shown.
Line ID 788F09
Locus At2g25900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11042015
Primers used for PCR BC67 - 8474, show primer details
   
Sequence Name 24-K104153-0022-788-F09C-BC67
GenBank Accession LN496967 [GenBank]
Sequencing primer gene specific primer, BC67: 5'-ATTGTGGATAGGGTCAAGATTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K104153-0022-788-F09C-BC67
AGCAGTTTATATCACAGACACACACCTCTCTCTCTTCTAGACTCACTAAACAAAAATGAT
GATCGGCGAAAATAAAAACCGGCCACATCCAACGATCCATATCCCTCAATGGGATCAAAT
CAACGATCCAACGGCCACAATCTCTTCACCATTCTCTTCCGTCAACCTTAACAGCGTTAA
CGACTACCCACACTCTCCGTCACCGTATCTCGACTCCTTCGCTTCTCTCTTCCGTTACCT
CCCGTCAAACGAGTTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGC
   
Sequence Name 24-K104154-0022-788-F09C-8409
GenBank Accession LN496968 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K104154-0022-788-F09C-8409
GCCATTTACATTGAATACTCGTTTGACGGGAGGTAACGGAAGAGAGAAGCGAAGGAGTCG
AGATACGGTGACGGAGAGTGTGGGTAGTCGTTAACGCTGTTAAGGTTGACGGAAGAGAAT
GGTGAAGAGATTGTGGCCGTTGGATCGTTGATTTGATCCCATTGAGGGATATGGATCGTT
GGATGTGGCCGGTTTTTATTTTCGCCGATCATCATTTTTGTTTAGTGAGTCTAGAAGAGA
GAGAGGTGTGTGTCTGTGATATAAACTGCTTTATTTTAACCATGGTTGGGTCAAATATTA
ACTACGGTAAAGTTATGAAAATCTTGACCCTATCCACA

Last Updated on 06.12.2018 16:11