Sequences from confirmation amplicon


Confirmation sequences for line 793F10 and hit At1g78880 are shown.
Line ID 793F10
Locus At1g78880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29653545
Primers used for PCR NF34 - 8474, show primer details
   
Sequence Name 38-K102431-0022-793-F10C-8409
GenBank Accession LN502929 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K102431-0022-793-F10C-8409
TTACAATTGAATATATCCTGAAATGTAGAAGTCTACCACATGCCTCTGCAATGTATTGTA
GGAGTAACGTTTATGCATTTAGAGACATTCCACACTGAAAGAGACCATTTTTCTGTGAAA
TGAAATATCATCATGCCACTACTAACCTCTGCTGATCTCAGTCCCCATGTAAAGCGACGA
TGTGAAGCATTGGCTGGCTTTGAACCCCATCCACGGTACTCATATAGACAAGTAGACGTG
TAAACACACCTAGGAACTCTATGGAAGGATGATTCAAGAGGCACGTTACCACAGGTGACC
ACCTTTTCAATGAACAAAATATAAAAACCAATATTATGAACATAGTAGAGATACCAAAGG
AAGGAAGCCAAATCACATTTCGTCATTTCCCATGATAAAGCAGTAGCACATAGTAAATGC
AAAACACCCAGGATGCATAGATACTTACCCCAGTGACCTTCACATATTGACCGTTTTTGG
CAGTTCTAAGATCAGCATCCGGATAACGAGCGATGAAATCTGTGATACCACGTCTTTCAC
AAGAAATATTCCAAATGAAAAGTGCAGCAACAACTGTGAACAAGACCGCCACAACTATGA
GGAGAATTGCATTGTGAAAGACGTGGTATCACAGATTTCATCGCTCGTTATCCGGATGCT
GNTCTTAGAACTGCC
   
Sequence Name 38-K102432-0022-793-F10C-NF34
GenBank Accession LN502930 [GenBank]
Sequencing primer gene specific primer, NF34: 5'-TTCACAATGCAATTCTCCTCATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K102432-0022-793-F10C-NF34
TGCACTTTTCATTTGGAATATTTCTTGTGAAAGACGTGGTATCACAGATTTCATCGCTCG
TTATCCGGATGCTGATCTTAGAACTGCCAAAAACGGTCAATATGTGAAGGTCACTGGGGT
AAGTATCTATGCATCCTGGGTGTTTTGCATTTACTATGTGCTACTGCTTTATCATGGGAA
ATGACGAAATGTGATTTGGCTTCCTTCCTTTGGTATCTCTACTATGTTCATAATATTGGT
TTTTATATTTTGTTCATTGAAAAGGTGGTCACCTGTGGTAACGTGCCTCTTGAATCATCC
TTCCATAGAGTTCCTAGGTGTGTTTACACGTCTACTTGTCTATATGAGTACCGTGGATGG
GGTTCAAAGCCAGCCAATGCTTCACATCGTCGCTTTACATGGGGACTGAGATCAGCAGAG
GTTAGTAGTGGCATGATGATATTTCATTTCACAGAAAAATGGTCTCTTTCAGTGTGGAAT
GTCTCTAAATGCATAAACGTTACTCCTACAATACATTGCAGAGGCATGTGGTAGACTTCT
ACATTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11