Sequences from confirmation amplicon


Confirmation sequences for line 795G03 and hit At3g57130 are shown.
Line ID 795G03
Locus At3g57130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21148199
Primers used for PCR AC46 - 8474, show primer details
   
Sequence Name 06-K028094-022-795-G03C-8409
GenBank Accession LN488665 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K028094-022-795-G03C-8409
ATTTCATTGAATTCTCCACAATTCGATCTTGGCTCGTGCTTATGTGGTACAATTGAGACT
TGACCACTGTACAAGAATTGAAGTAGAAGTAAGAAAACTTCGTAACCGACTGAGTTAACC
GGTATGACTCCTCCTACTGCGGCTGCTCTGGCTCCTGATCCGGTTTGGTTAGCGGGTTCA
GCTCCGGGTTGCGAAGGATCAGATTCACAGAAGAATTTGCGGAAGAAGAGGCTACGAGCA
GCGAGGATGCAACGGTGAGCGTGGACTAGACGGCCTTCGACGCTGAAAGTGACATCGGAG
AAAGCTTGACCGTTGATTAAGAGGTTTAGGTAATCCAATGACATAGATTTGAGTGATTCT
TCGAAAGTATTGCTCATAGCTCCTATTGTTG
   
Sequence Name 06-K028094-022-795-G03C-AC46
GenBank Accession LN488666 [GenBank]
Sequencing primer gene specific primer, AC46: 5'-ATCAACAAAGGAGCTATGAGCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K028094-022-795-G03C-ac46
TTGGATACCTAAACCTCTTAATCACGGTCAAGCTTTCTCCGATGTCACTTTCAGCGTCGA
AGGCCGTCTAGTCCACGCTCACCGTTGCATCCTCGCTGCTCGTAGCCTCTTCTTCCGCAA
ATTCTTCTGTGAATCTGATCCTTCGCAACCCGGAGCTGAACCCGCTAACCAAACCGGATC
AGGAGCCAGAGCAGCCGCAGTAGGAGGAGTCATACCGGTTAACTCAGTCGGTTACGAAGT
TTTCTTACTTCTACTTCAATTCTTGTACAGTGGTCAAGTCTCAATTGTACCACATAAGCA
CGAGCCAAGATCGAATTGTGGAGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGG

Last Updated on 06.12.2018 16:11