Sequences from confirmation amplicon


Confirmation sequences for line 797B09 and hit At4g20140 are shown.
Line ID 797B09
Locus At4g20140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10886369
Primers used for PCR EU70 - 8474, show primer details
   
Sequence Name 77-K103762-0022-797-B09C-EU70
GenBank Accession LN513538 [GenBank]
Sequencing primer gene specific primer, EU70: 5'-GAACCTCACCAGAATCAATCTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K103762-0022-797-B09C-EU70
ATTATCCATTATGTGGTTCGAGCTCATATCTCTCATTCGATGTCACCAACCATTGATTTG
AAGATGAAATCCCCCTGGAGCTAGGAAACTCTCAAAATCTTGATCGTCTCAGGTTAGGCA
AGAATCAATTGACAGGAAAAATCCCTTGGACGTTGGGGAAAATCCGCGAGCTGTCCTTGT
TAGATATGTCAAGCAACGCTCTCACAGGAACCATTCCGCTACAGCTCGTCTTGTGCAAGA
AACTGACACACATCGATCTCAATAACAATTTTCTATCAGGTCCTATACCTCCATGGCTTG
GAAAACTTTCACAGTTAGGAGAGCTCAAGCTTTCTTCGAATCAGTTTGTTGAGTCTTTAC
CTACTGAACTGTTCAACTGTACAAAGCTGCTTGTGTTGTCTCTTGATGGAAATTCGCTTA
ATGGGTCGATTCCACAAGAGATTGGTAAATGAGTAAATGGCTTCACCATTGTATCAGCAA
TGAGTATGATGGTGAATGAGTAAATGGCTTCACCATGGTATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTA
   
Sequence Name 68-K103763-0022-797-B09C-8409
GenBank Accession LN511999 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K103763-0022-797-B09C-8409
GGTTCAATCGAATGAGAGATATGAGCTCGAACCACATAATGGATGAATCGTACCATTGAG
CCTGTTGTGAGAGAGATTGATTCTGGTGAGGTT

Last Updated on 06.12.2018 16:11