Sequences from confirmation amplicon


Confirmation sequences for line 799H09 and hit At5g13180 are shown.
Line ID 799H09
Locus At5g13180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4197479
Primers used for PCR DB27 - 8474, show primer details
   
Sequence Name 54-K029917-022-799-H09C-8409
GenBank Accession LN508368 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K029917-022-799-H09C-8409
ATCTCAATTTGGTCACTCGAATTGTTATTATTATTAATCGTCTAAGAATTACGGCTATCT
CCGTCGTCGTCGTCGTTCTTAGTTACCGGCTCTTTTCTTCAAGAAGATACGACAGAGTAC
CCAGTTCTGAGTGGGACCCATAGAACTCTACAAAATATTAGAATCTTATTTTAGTGGTGG
TGATTAAACCGGAGCTTAAACCGGGAATTGAGAGAGTGGGAATATGTACCGGAGGAGAAG
AAGAGAGGCGATATTCGTGCATGATCCAATCGGTTCTTGAGCCATGAGGTGGTTTGCCTT
TGTAGAAGACAAGAGTTTTCTTCAAACCAACGATT
   
Sequence Name 54-K029917-022-799-H09C-DB27
GenBank Accession LN508369 [GenBank]
Sequencing primer gene specific primer, DB27: 5'-CTCTAGAGGAAATCAAATCGTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K029917-022-799-H09C-DB27
GGCAACACCTCTGGCTCAGAACCGATTGGATCATGCACGAATATCGCCTCTCTTCTTCTC
CTCCGGTACATATTCCCACTCTCTCAATTCCCGNGTTTAAGCTCCGGTTTAATCACCACC
ACTAAAATAAGATTCTAATATTTTGTAGAGTTCTATGGGTCCCACTCAGAACTGGGTACT
CTGTCGTATCTTCTTGAAGAAAAGAGCCGGTAACAAGAACGACGACGACGACGGAGATAG
CCGTAATCTTAGACATAATAATAATAACAATTCGAGTGACCAAATTGAGATAATTACAAC
AGACCAAACAGATGATAAAACAAAACCAATCTTCTTTGATTTCATGAGAAAAGAAAGAAC
AACAGATTTGAACCTTTTGCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACG
ACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGA
AATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11