Sequences from confirmation amplicon


Confirmation sequences for line 800H03 and hit At1g51950 are shown.
Line ID 800H03
Locus At1g51950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19306730
Primers used for PCR BC04 - 8474, show primer details
   
Sequence Name 42-K103981-0022-800-H03C-BC04
GenBank Accession LN504439 [GenBank]
Sequencing primer gene specific primer, BC04: 5'-TGCCCTAGTTTTCCTCTGTTTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K103981-0022-800-H03C-BC04
AGTTTTGTTTTGATGCCTTTGTGTTTTAAAGAACTGCTCCCGGTCCAGTGGTGGGATGGC
CTCCGGTTCGATCCTTCAGGAAGAATTTAGCTAGTGGAAGTTCTTCAAAGCTTGGAAATG
ACTCAACCACCTCCAATGGTGTTACCCTCAAGAACCAAAAGTGTGATGCTGCTGCTAAGA
CAACAGAACCAAAGAGACAAGGAGGCATGTTTGTGAAGATCAACATGTATGGTGTTCCCA
TTGGTCGTAAAGTTGATCTCAGTGCTCATAATAGCTATGAGCAGTTATCTTTCACAGTTG
ACAAGCTCTTCAGAGGTCTTCTTGCAGGTAAATTTGAAACCTTTGTCTCTCTATTGATTA
TGGTTAAAAAAGGGTTGAATTTTGAACTCTAATTGTTCTTATTTTATCAGCTCAAAGAGA
CTTTCCATCATCCATAGAAGATGAAAAACCAATCACTGGATTATTAGATGGGAATGGAGA
ATATACTCTTACATATGAAGACAATGAAGGAGACAAGATGCTTGTAGGAGATGTTCCATG
GCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGT
   
Sequence Name 58-K103982-0022-800-H03C-8409
GenBank Accession LN509688 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K103982-0022-800-H03C-8409
GCCATTTACATTGAATATATCCTGCCATGGAACATCTCCTACACGTATCTTGTCTCCTTC
ATTGTCTTCATATGTAAGAGTATATTCTCCATTCCCATCTAATAATCCAGTGATTGGTTT
TTCATCTTCTATGGATGATGGAAAGTCTCTTTGAGCTGATAAAATAAGAACAATTAGAGT
TCAAAATTCAACCCTTTTTTAACCATAATCAATAGAGAGACAAAGGTTTCAAATTTACCT
GCAAGAAGACCTCTGAAGAGCTTGTCAACTGTGAAAGATAACTGCTCATAGCTATTATGA
GCACTGAGATCAACTTTACGACCAATGGGAACACCATACATGTTGATCTTCACAAACATG
CCTCCTTGTCTCTTTGGTTCTGTTGTCTTAGCAGCAGCATCACACTTTTGGTTCTTGAGG
GTAACACCATTGGAGGTGGTTGAGTCATTTCCAAGCTTTGAAGAACTTCCACTAGCTAAA
TTCTTCCTGAAGGATCGAACCGGAGGCCATCCCACCACTGGACCAGGAGCAGTTCTTTAA
AACACAAAGGCATCAAAACAAAACTTGTTAGTAAGACAAGAACAATCTCAAGGTTAAAAA
CAGAGG

Last Updated on 06.12.2018 16:11