Sequences from confirmation amplicon


Confirmation sequences for line 804D10 and hit T20L15 are shown.
Line ID 804D10
Locus T20L15 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:324115
Primers used for PCR AC32 - 8474, show primer details
   
Sequence Name 27-K027917-022-804-D10C-8409
GenBank Accession LN498238 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K027917-022-804-D10C-8409
GCCATTTACACTATCGATGATACAACTTTTTATATCATCGTGGTGCACATTTTAAAAGGA
TTACAATGACCACGATTAAACTAGTAATTAGTACATATACAAACTAGTAATTACTCTATT
ATTTATTTCTTTGGTACGAACACTACTTGTGTATTACTATTACTAAAACATATAATAAAA
GTGTCAGTGTCAAATGTCAATGTAATTTAATTTAGGGGAAAATGAAATGGCCAACATAAT
CTAACACAAAATCAATGAAAATGGAGAGAGCATCTCACTCACCTTATAATAACCTTTATA
TTTTCCACGTATGTGGGCCCCCTCACATAAACCTCTCTTTCCTTTATAAATTCTCCACTC
TTCACACTCTCACTTTTGTTCATGTACCAAAGAAGTCCCCAACAAACACAAAATAATCAA
CAGAGAGAGTCAGAGAGATAATGGGTAATAACTATCGGTTTAAGCTATCGGAGT
   
Sequence Name 28-K027917-022-804-D10C-AC32
GenBank Accession LN498702 [GenBank]
Sequencing primer gene specific primer, AC32: 5'-AAAACCAAGCGTTAGGGATTAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K027917-022-804-D10C-AC32
CCCATTATCTCTCTGACTCTCTCTGTTGATTATTTTGTGTTTGTTGGGGACTTCTTTGGT
ACATGAACAAAAGTGAGAGTGTGAAGAGTGGAGAATTTATAAAGGAAAGAGAGGTTTATG
TGAGGGGGCCCACATACGTGGAAAATATAAAGGTTATTATAAGGTGAGTGAGATGCTCTC
TCCATTTTCATTGATTTTGTGTTAGATTATGTTGGCCATTTCATTTTCCCCTAAATTAAA
TTACATTGACATTTGACACTGACACTTTTATTATATGTTTTAGTAATAGTAATACACAAG
TAGTGTTCGTACCAAAGAAATAAATAATAGAGTAATTACTAGTTTGTATATGTACTAATT
ACTAGTTTAATCGTGGTCATTGTAATCCTTTTAAAATGTGCACCACGATGATATTAAAAA
GTTGTATCATCGATAGTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11