Sequences from confirmation amplicon


Confirmation sequences for line 807G12 and hit At3g04710 are shown.
Line ID 807G12
Locus At3g04710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1280546
Primers used for PCR IS76 - 8474, show primer details
   
Sequence Name 33-K026486-022-807-G12C-8409
GenBank Accession LN500848 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K026486-022-807-G12C-8409
TTTACAATTGAATATATCCATATTGAGCATCAGATAAGGCATGCTCAGCTTGTCCTAGTC
GCAACCAGCAGAGGCTTCGGTTTGAGAATAAGGTATGATCCGTTGGGTCAAAATCAATTG
CCTGTGTTCAAACAAACCACAAAATCACATATAGGCAAAAATTAAGAATCTTGTTTTGAT
TTCAGAAGAAGTTTTAGAGTAGAATACTTGTGTATAAGCATCAATAGCCATCTGAAAATC
CTTTCTGTGAAAGGCGTCTTGTCCTCTGGCTTTTGCTTCTGCGGCTTTTGCCTTTGCTTC
TGGGGAGACCTGTGGAAGTTAAAAATGGTTATCTTTAAGTACTTTAAACAATTTGTTGAA
AGAGAAATGCATATTGATCAAAGTATGAAGTACCACAGGAAGATCTTTCTTGATCCCAGT
CTCTTGGGATTTCGCCTTGTTTGAGTTTTCCTCTTGTTCCTGAAAATGTTAAGTTGTTTC
AGACAAAAAATATCGAAATATCCGACACTAATGCCAGGAAAGATCTGATATGCCTACTTT
GTTTGATTCC
   
Sequence Name 34-K026486-022-807-G12C-IS76
GenBank Accession LN501270 [GenBank]
Sequencing primer gene specific primer, IS76: 5'-AGTAGATGGAATTCTCGCTCACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K026486-022-807-G12C-IS76
CAAACAAAGTACGCATATCAGATCTTTCCTGGCATTAGTGTCGGATATTTCGATATTTTT
TGTCTGAAACAACTTAACATTTTCAGGAACAAGAGGAAAACTCAAACAAGGCGAAATCCC
AAGAGACGGGGATCAAAAAAAATCTTCCGGGGGAACTTCATACTTTGATCAATATGCATT
TCTCTTTCAACAAATGGTTTAAAGTACTTAAAGATAACCATTTTTAACTTCCACAGGCCT
CCCCAAAAGCAAAGGCAAAAGCCGCAAAAGCAAAAGCCAGAGGCACAAGACGCCTTTCAC
AGAAAGGATTTTCAGATGGCTATTGATGCTTATACACAAGTATTCTACTCTAAAACTTCT
TCTGAAATCAAAACAAGATTCTAAATTTGCGCCTATATGAGATTTTGGGGTGTGTTAGAA
CACAGGCAATTGATTTTGACCCAACGGATCATACCTTATTCTCAAACCGAACCCTCTGCT
GGTTGCGACTAGGACAAGCTGAGCATGCCTTATCTGATGCTCAATATGGATATATTCAAT
TGAAAATGGCTTCATGTCCGGGAAATCTACGTGGAT

Last Updated on 06.12.2018 16:11