Sequences from confirmation amplicon


Confirmation sequences for line 812A10 and hit At2g02860 are shown.
Line ID 812A10
Locus At2g02860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:831580
Primers used for PCR AC37 - 8474, show primer details
   
Sequence Name 29-K027917-022-812-A10C-8409
GenBank Accession LN499148 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K027917-022-812-A10C-8409
CCATTTACAATTGATCCATTTACATTGCTGAAGAACCCTTTTGAGTTTTATCTAACAGTG
TTGCATTTGGTTGGTTGATGCAGACCCTTGGAATATCGCATGCTTTTTCTTCGTTTATTT
GGCTGTGCGGCCCAATTACAGGCCTTGTGGTAGGTTAAAAGTTTCTTTAAGAATTTTAGC
AGTTTTACTATCTTGCACGTAATGATCTCACGAGAGTTCAAGTTTTGATTTATATTGGAT
CAGAAACTATTTTTCTGCCCCATTTTGTTCATACTTATTTGTTTGTTGGGTTTTAGGTCC
AGCCTTTTGTTGGCATTTGGAGTGATAAATGTACTTCAAAGTATGGAAGAAGACGACCAT
TTATTCTAGTTGGATCATTCATGATCTCAATAGCAGTAAGTGCCAATAGAAGATATGATT
TCTGTTTTGATTTTCTTTTAGGAGATAG
   
Sequence Name 30-K027917-022-812-A10C-AC37
GenBank Accession LN499583 [GenBank]
Sequencing primer gene specific primer, AC37: 5'-ACACAATGTAGCCAGCTATCTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K027917-022-812-A10C-AC37
ATATCTTCTATTGGCACTTACTGCTATTGAGATCATGAATGATCCAACTAGAACAAATGG
TCGTCTTCTTCCATACTTTGAAGTACATTTATCACTCCAAATGCCAACAAAAGGCTGGAC
CTAAAACCCAACAAACAAATAAGTATGAACAAAATGGGGCAGAAAAATAGTTTCTGATCC
AATATAAATCAAAACTTGAACTCTCGTGAGATCATTACGTGCAAGATAGTAAAACTGCTA
AAATTCTTAAAGAAACTTTTAACCTACCACAAGGCCTGTAATTGGGCCGCACAGCCAAAT
AAACGAAGAAAAAGCATGCGATATTCCAAGGGTCTGCATCAACCAACCAAATGCAACACT
GTTAGATAAAACTCAAAAGGGTTCTTCAGCAATGTAAATGGATCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTATTTC

Last Updated on 06.12.2018 16:11