Sequences from confirmation amplicon


Confirmation sequences for line 814G03 and hit At1g76550 are shown.
Line ID 814G03
Locus At1g76550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28725414
Primers used for PCR NA56 - 8474, show primer details
   
Sequence Name 83-K101673-0022-814-G03C-8409
GenBank Accession LN514287 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K101673-0022-814-G03C-8409
CGGAATGAGCCATTTACATTGAATATATCCTGTTGAGCATTTTGCTTCACAAAAAATTCA
GCAAGATGAGCGGCATCTGTGTTTGATGTTACACCTAACAAATAAAAACATTGATTTAGA
AAATTCTGGGATGAACTTAAAAAATCATATTTACACAAGTTAAGAACAAAACATCTACTA
AAACAGACACCCGAAAAAGAGGAAAAGTAGGTACCTCCAATGATAACTAGGCTATCCAAT
TTCAAATCCGTGCATGCTTTTAGTGCAGCATTGACCTGTTCGGTTGTTCTAATCTGATCC
TTGGTTCTTCCAAGCATATCATAGCCACCTAATACGACATACATTTAAATGCCATATGTC
AGAAAGCAGCAGAGATAAAGTTCTGAATAATCTTAATCAAACCACCAACCACCAACCTTG
GTTTTTGTAAGTTTGGAGGACCTCATCAGTGATCTCCAGGGTCTTCTGGGCAAAGAGACC
TTCAGAACCACCTGTTACAGGTAGCCNAAGTTTCATATTAATGACCAGCACCACAT
   
Sequence Name 83-K101680-0022-814-G03C-NA56
GenBank Accession LN514288 [GenBank]
Sequencing primer gene specific primer, NA56: 5'-GATGTGTGCCTGGTCATTAATATG-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K101680-0022-814-G03C-NA56
TGAGGTCTCTTTGCCCGAAGACCCTGGAGATCACTGATGAGGTCCTCCAAACTTACAAAA
ACCAAGGTTGGTGGTTGGTGGTTTGATTAAGATTATTCAGAACTTTATCTCTGCTGCTTT
CTGACATATGGCATTTAAATGTATGTCGTATTAGGTGGCTATGATATGCTTGGAAGAACC
AAGGATCAGATTAGAACAACCGAACAGGTCAATGCTGCACTAAAAGCATGCACGGATTTG
AAATTGGATAGCCTAGTTATCATTGGAGGTACCTACTTTTCCTCTTTTTCGGGTGTCTGT
TTTAGTAGATGTTTTGTTCTTAACTTGTGTAAATATGATTTTTTAAGTTCATCCCAGAAT
TTTCTAAATCAATGTTTTTATTTGTTAGGTGTAACATCAAACACAGATGCCGCTCATCTT
GCTGAATTTTTTGCTGAAGCAAAATGCTCAACAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11