Sequences from confirmation amplicon


Confirmation sequences for line 815G11 and hit At1g08060 are shown.
Line ID 815G11
Locus At1g08060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2507959
Primers used for PCR HM89 - 8474, show primer details
   
Sequence Name 18-K101563-0022-815-G11C-8409
GenBank Accession LN494241 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K101563-0022-815-G11C-8409
GCCATTTACAATTGAATATAAAAAGCATACTTTCATCCCACTCAAATTTCAATACAAGAC
AGAATTCCATTGTCAAAAAGAAAATGCACATATATTGAGAATGCACAAAGTGACGTATTC
ATGACAAACATAGAGCTATTCACATTCGCAAGTAATAGAAAATGCACCTGGTAGAAGACA
ACTGCTTTTAAACCATTCTTTTTTATATGAGTAAGCATTTTATCAAGGAGGTGAAGTTTC
CCGCTCGCTTTAATTTCTACATCCAGGATTTCATGCAACTCCAGATTCTTGGTGAGCAGT
TGTTTCAAAGATGCATCCATAACGTATGGATGGTCACAGGTCTGTCAACAAGTTGCAAAC
ACATAAAGAAGATTTTAATATATTCACACATTAACATGCAGAAAGAGGCCTTGAGAAAAT
GTGATGCTGACCGACAATAGTCGTATATCTNAGCAACCCCAA
   
Sequence Name 18-K101576-0022-815-G11C-HM89
GenBank Accession LN494242 [GenBank]
Sequencing primer gene specific primer, HM89: 5'-TTTGGGGTTGCTTAGATATACGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K101576-0022-815-G11C-HM89
TTTTCTAGGCCTCTTTCTGCATGTAATGTGTGAATATATTAAAATCTTCTTTATGTGTTT
GCAACTTGTTGACAGACCTGTGACCATCCATACGTTATGGATGCATCTTTGAAACAACTG
CTCACCAAGAATCTGGAGTTGCATGAAATCCTGGATGTAGAAATTAAAGCGAGCGGGAAA
CTTCACCTCCTTGATAAAATGCTTACTCATATAAAAAAGAATGGTTTAAAAGCAGTTGTC
TTCTACCAGGTGCATTTTCTATTACTTGCGAATGTGAATAGCTCTATGTTTGTCATGAAT
ACGTCACTTTGTGCATTCTCAATATATGTGCATTTTCTTTTTGACAATGGAATTCTGTCT
TGTATTGAAATTTGAGTGGGATGAAAGTATGCTTTTTATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11