Sequences from confirmation amplicon


Confirmation sequences for line 817A02 and hit At2g02450 are shown.
Line ID 817A02
Locus At2g02450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:647765
Primers used for PCR PF38 - 8474, show primer details
   
Sequence Name 05-K103004-0022-817-A02C-8409
GenBank Accession LN488308 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K103004-0022-817-A02C-8409
CCATTTACATTGAATATATTCTCAATTTGACAAGTCTTTGTCTCCTCCTTAACTCTCTTC
TTCTCTCTCTTTGTCTTATTCTAGTTTGGTCTCAACTCCCAAAAACGATGACGTTTCATT
CCTATATAGTCGTCCTCTCTTCTTCTTCTCCTCCTCTTATCTCTACTCAACACCACTCAA
TTTCATCTCTCTTTTTCCCTTCCATTGTTAGTATAAAAACCAAGCAAACCCTTAATCACT
TTTCATCATCATATATCACCTTAATCCACATGCATACACATATCTAGTCTTTTTGATATA
TGGCAATTGTATCCTCCACAACAAGCATCATTCCCATGAGTAACCAAGTCAACAATAACG
AAAAAGGTATAGAAGACAATGATCATAGAGGCGGCCAAGAGAGTCATGTCCAAAATGAAG
ATGAAGCTGATGATCATGATCATGACATGGTCATGCCCGGATTTAGATTCCATCCTACCG
AAGAAGAACTCATAGAGTTTTACCTTCGCCGAAAAGTTGAAGGCAAACGCTTTAATGTAG
AACTCATCACTTTCCTCGATCTTTATCGCTATGATCCTTGGGAACTTCCTGGTAAATATA
CATTCACATAAACACACATAAATCATCTCAAACTATTTGGAAATCTTAATTTCTATTCAT
ATGTTAAGATCTTTCTTCTCTCTNATCACTTTCTCTCTCTATTTCTTTTTTTTTAACCTA
TATATGTACCTACCTCCTTATGAAGTATTACTATGTCGATCGTTAACAATTCTCAATATC
TTTAAACGCTTCTCCCTCT
   
Sequence Name 53-K103005-0022-817-A02C-PF38
GenBank Accession LN508118 [GenBank]
Sequencing primer gene specific primer, PF38: 5'-AAAGAAACTAAAGAGGGAGAAGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K103005-0022-817-A02C-PF38
TGTTACGATCGAATAGTAATACTTCATAAGGAGGTAGGTACATATATAGGTTAAAAAAAA
AGAAATAGAGAGAGAAAGTGATAAGAGAGAAGAAAGATCTTAACATATGAATAGAAATTA
AGATTTCCAAATAGTTTGAGATGATTTATGTGTGTTTATGTGAATGTATATTTACCAGGA
AGTTCCCAAGGATCATAGCGATAAAGATCGAGGAAAGTGATGAGTTCTACATTAAAGCGT
TTGCCTTCAACTTTTCGGCGAAGGTAAAACTCTATGAGTTCTTCTTCGGTAGGATGGAAT
CTAAATCCGGGCATGACCATGTCATGATCATGATCATCAGCTTCATCTTCATTTTGGACA
TGACTCTCTTGGCCGCCTCTATGATCATTGTCTTCTATACCTTTTTCGTTATTGTTGACT
TGGTTACTCATGGGAATGATGCTTGTTGTGGAGGATACAATTGCCATATATCAAAAAGAC
TAGATATGTGTATGCATGTGGATTAAGGTGATATATGATGATGAAAAGTGATTAAGGGTT
TGCTTGGTTTTTATACTAACAATGGAAGGGAAAAAGAGAGATGAAATTGAGTGGTGTTGA
GTAGAGATAAGAGGAGGAGAAGAAGAAGAGAGGACGACTATATAGGAATGAAACGTCATC
GTTTTTGGGAGTTGAGACCAAACTAGAATAAGACAAAGAGAGAGAAGAAGAGAGTTAAGG
AGGAGACAAAGACTTGTCAAATTGAGAATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAA

Last Updated on 06.12.2018 16:11