Sequences from confirmation amplicon


Confirmation sequences for line 819B03 and hit At5g17420 are shown.
Line ID 819B03
Locus At5g17420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5739228
Primers used for PCR NK56 - 8474, show primer details
   
Sequence Name 12-K103431-0022-819-B03C-NK56
GenBank Accession LN491538 [GenBank]
Sequencing primer gene specific primer, NK56: 5'-AGACACGGAGATCAGTTTCTTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K103431-0022-819-B03C-NK56
TACCAGGGCATTCATGGCGCCAGCTTTCTTGTGGTGTTGAAAACCAGGACGCTTCTCACG
GGACACGTACACAAGCCGAGGAAGCTCATGCCCTTCGACATCAAATCCTCCGCTGTGGCC
GAGGAAGACTTGGATCATACCGGGGTGGTCCTTGGTGTTGTTCCCTGGCCACGGTGTTCC
ATCTTGCATGATCCAACCTTCTAGAGGAACCTTTGAGGCCTTCGCCACTTGAGCGTTGAT
CCTGACCTTGAACTCCTCATATTCTCTCTGTCATATGTCAATTTCACTTATGAAGTTTAG
ATTATAGTGAAAAGATGGGTGGTGGTATAACTAGATCAGGTGATATACACTGACCTTCAT
GGCTCGACGTTCCTTAACAAATGTTGGGTGGATCAGCAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 28-K103432-0022-819-B03C-8409
GenBank Accession LN498804 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K103432-0022-819-B03C-8409
GCCATTGCTGATCCACCCAACATTTGTTAAGGAACGTCGAGCCATGAAGGTCAGTGTATA
TCACCTGATCTAGTTATACCACCACCCATCTTTTCACTATAATCTAAACTTCATAAGTGA
AATTGACATATGACAGAGAGAATATGAGGAGTTCAAGGTCAGGATCAACGCTCAAGTGGC
GAAGGCCTCAAAGGTTCCTCTAGAAGGTTGGATCATGCAAGATGGAACACCGTGGCCAGG
GAACAACACCAAGGACCACCCCGGTATGATCCAAGTCTTCCTCGGCCACAGCGGAGGATT
TGATGTCGAAGGGCATGAGCTTCCTCGGCTTGTGTACGTGTCCCGTGAGAAGCGTCCTGG
TTTTCAACACCACAAGAAAGCTGGCGCCATGAATGCCCTGGTAATTTTCTTGATCTGCCT
TGGACCAAACAAGAAACTGATCTCCCGTGT

Last Updated on 06.12.2018 16:11