Sequences from confirmation amplicon


Confirmation sequences for line 821C08 and hit At1g74160 are shown.
Line ID 821C08
Locus At1g74160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27888615
Primers used for PCR 37dl - 8474, show primer details
   
Sequence Name 61-K110039-0022-821-C08C-37dl
GenBank Accession LN510546 [GenBank]
Sequencing primer gene specific primer, 37dl: 5'-CTTATCAAACGAGCTGGTACATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K110039-0022-821-C08C-37dl
CTTTTGTCACCCGTTTACTATTACTTGCTGAAGTTCCTTTATCATCAGGTTTTTCTCTAC
GGATCTTCTTGATGCCAGTTAGGCTGTGAATGGGAATAAGGGATGACGCAGGAATACCAG
CCTTCTCAACAAGCTTGGCAGGTTTCATAATCACAATCGGGGATTGATATACCTGGTTAG
ATGAAGAAGACTGAACTCTCGTTCTGGATGACATTGCATGGTTGGAAGTTGCAGAATTTT
CTCTTTCATAATCTCTTTGAACTGCAAAGTTTGTGGATTGTTGTTGTTTCTCAGTATCTA
GGAAACCTTTTGACTGCATAGACTCTAATATTTGTTTAAGGGCTCGCAGATCTTTTCCAG
AATGTTTGAACTCAAGATCATTCAATCTCCGCTCCATTTCACTGTACACAGTAGGAGGAA
AATTAGGTGCTTCATACGGCTTCGCTTTCACAGGCATGCTAGCTTGCTTTTGCAAAACTC
GGTTTCTATCAGCGTGTTTCCATGGAGCTGGTTCAACGGGGAATCTTGTATTTGATAGAG
GTTTCATAACAAAATCAGAGTTTCTCCATCTTGGAGAAGCCGGGTCCTTCCCCAAGCTTC
TCGGTGAGCTAGGAGAAAAACGGATTGCACGGTTCAGATTTTTCTCCCTCAATGATCTTG
AGAATGGATCGTCATTTTGGTCAGAGATGTTGGTTTTGTTCAGACCAAACTGATGAATGT
CTCTACCCAATGGAGAACCGGGCAATGTCTCCAATTAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
AGAAGAGTAATTACCAATTTTTTTTCAATTCAAAAT
   
Sequence Name 45-K110038-0022-821-C08C-8409
GenBank Accession LN505559 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K110038-0022-821-C08C-8409
AAGCCATTTACAATTGAATATATCCTAATTGGAGACATTGCCCGGTTCTCCATTGGGTAG
AGACATTCATCAGTTTGGTCTGAACAAAACCAACATCTCTGACCAAAATGACGATCCATT
CTCAAGATCATTGAGGGAGAAAAATCTGAACCGTGCAATCCGTTTTTCTCCTAGCTCACC
GAGAAGCTTGGGGAAGGACCCGGCTTCTCCAAGATGGAGAAACTCTGATTTTGTTATGAA
ACCTCTATCAAATACAAGATTCCCCGTTGAACCAGCTCCATGGAAACACGCTGATAGAAA
CCGAGTTTTGCAAAAGCAAGCTAGCATGCCTGTGAAAGCGAAGCCGTATGAAGCACCTAA
TTTTCCTCCTACTGTGTACAGTGAAATGGAGCGGAGATTGAATGATCTTGAGTTCAAACA
TTCTGGAAAAGATCTGCGAGCCCTTAAACAAATATTAGAGTCTATGCAGTCAAAAGGTTT
CCTAGATACTGAGAAACAACAACAATCCACAAACTTTGCAGTTCAAAGAGATTATGAAAG
AGAAAATTCTGCAACTTCCAACCATGCAATGTCATCCAGAACGAGAGTTCAGTCTTCTTC
ATCTAACCAGGTATATCAATCCCCGATTGTGATTATGAAACCTGCCAAGCTTGTTGAGAA
GGCTGGTATTCCTGCGTCATCCCTTATTCCCATTCACAGCCTAACTGGCATCAAGAAGAT
CCGTAGAGAAAAACCTGATGATAAAGGAACTTCAGCAAGTAATAGTAAACGGGTGACAAA
AGATTGTAGTCCTGGAAACGAAGAGCTGAATCATGTAC

Last Updated on 06.12.2018 16:11