Sequences from confirmation amplicon


Confirmation sequences for line 821F04 and hit At1g14520 are shown.
Line ID 821F04
Locus At1g14520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4969016
Primers used for PCR MA70 - 8474, show primer details
   
Sequence Name 82-K102437-0022-821-F04C-8409
GenBank Accession LN514175 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K102437-0022-821-F04C-8409
TTTACATTGAATATATTTACAATTGATAACCCGAGTTACAACTCTAAGTATGGGATATAC
ACTGAAGGCTGTGGGCTCGACAATGTGTTAATGTCTTGGGGACATGATGATTACATGTAC
CTTGTAAGTTCATTACAATCTCAACATGTGTTTATGATTTACCGACATGTAAGAATAATC
TATATGATGTACATAACTGAATTTCACAGGTTGCGAAGGAGAATCAAACTACTTTACCAT
CAGCCGGTCTTTTCATCATCAGATACCATTCGTTCTACGGTATTTGAAACACAAAGACTG
ATTTGCTTGAATTTATTGGTTAATAGATGAGTCTAGATTATAAAACTACGGGTTATTGTT
ATTGTTTTTGGTTGGC
   
Sequence Name 82-K102438-0022-821-F04C-MA70
GenBank Accession LN514176 [GenBank]
Sequencing primer gene specific primer, MA70: 5'-TGCCAACCAAAAACAATAACAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K102438-0022-821-F04C-MA70
TCTATAACCATAAATTCAGCAAATCAGTCTTTGTGTTTCAAATACCGTAGAACGAATGGT
ATCTGATGATGAAAAGACCGGCTGATGGTAAAGTAGTTTGATTCTCCTTCGCAACCTGTG
AAATTCAGTTATGTACATCATATAGATTATTCTTACATGTCGGTAAATCATAAACACATG
TTGAGATTGTAATGAACTTACAAGGTACATGTAATCATCATGTCCCCAAGACATTAACAC
ATTGTCGAGCCCACAGCCTTCAGTGTATATCCCATACTTAGAGTTGTAACTCGGGTTATC
AATTGTAAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC
TTTATTTCGACGTGTCTACATTCACTCCCAAATGG

Last Updated on 06.12.2018 16:11