Sequences from confirmation amplicon


Confirmation sequences for line 827D09 and hit At1g72970 are shown.
Line ID 827D09
Locus At1g72970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27452731
Primers used for PCR GC56 - 8474, show primer details
   
Sequence Name 26-K033541-022-827-D09C-GC56
GenBank Accession LN497818 [GenBank]
Sequencing primer gene specific primer, GC56: 5'-ATTTCAATTTGCAACAAACAATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K033541-022-827-D09C-GC56
AGTTACTAGCTGGTAAAATAAATTAGTAGGTAACTATTTTTTATGAGTAAATGAGGGAAC
TATTAATTGAGTAAGATGATTCATATGTCCTTTTCATTTGCTATATTCGACAGAATGGGA
CACACCTAAAATCAAATTTACGTTTTAGTGTAGACCACCCCAACATCTCTTTATTACACA
ACTTGTGTTTTTTTTTACTATATGATTTTTTTACCGAATGTTGTTGTCTAACCAAAATTC
AATGTACCGTATAAGACTATAAGTTATACAACTCGTAGAAATGGTTACATATATGTAACA
ATATATAGAGACACATGTGGGTGTAATTAATATAGTTGCTCTGTTGGATCGTTAGTGTTA
TTTATAGATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
ACATTCACTC
   
Sequence Name 26-K033541-022-827-D09C-8409
GenBank Accession LN497817 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K033541-022-827-D09C-8409
CTAACGATCCAACAGAGCAACTATATTAATTACACCCACATGTGTCTCTATATATTGTTA
CATATATGTAACCATTTCTACGAGTTGTATAACTTATAGTCTTATACGGTACATTGAATT
TTGGTTAGACAACAACATTCGGTAAAAAAATCATATAGTAAAAAAAAACACAAGTTGTGT
AATAAAGAGATGTTGGGGTGGTCTACACTAAAACGTAAATTTGATTTTAGGTGTGTCCCA
TTCTGTCGAATATAGCAAATGAAAAGGACATATGAATCATCTTACTCAATTAATAGTTCC
CTCATTTACTCATAAAAAATAGTTACCTACTAATTTATTTTACCAGCTAGTAACTTTCCT
CACATTAGACTTATTGTTACAGAATTGTTTGTTGCNAATTGAAAT

Last Updated on 06.12.2018 16:11