Sequences from confirmation amplicon


Confirmation sequences for line 833C07 and hit At1g51600 are shown.
Line ID 833C07
Locus At1g51600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19134883
Primers used for PCR IF40 - 8474, show primer details
   
Sequence Name 87-K101387-0022-833-C07C-8409
GenBank Accession LN514741 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K101387-0022-833-C07C-8409
ATTTACATTGAATATATCCTGTCCGGGAAATGGCTTCATGTCCGGGAAACAAGGCAAGAC
AATACTTGCTTCAGGATACTCATTGCTGATCCATGAAGCCACGTGGACTTATGTGGGCAA
ACAAGGCAAGACAATACTTGCTTCTTAATTTGATCAACTCTCTTTATCTTGCTCATTTTG
TAGCACTAGTGATTCGATGATTAGTCTGTCTAAATGAGGACGACATTTATACTTTCAGGG
TGCTTTTAGGGACTTATCCAAAGCCTCTCCTCAAACAGCCCAGAATCTTCCTTTAAATAA
GAATGAAGTAAGCAACATCATGTTTTGACCAAACAATTTGTTGTACGAAAAGGAATCCAT
TCGTCTTTATCATAACTTGTGCATGTCTGATCTATCTTTGCAGGAATGCAATCTTGAGAC
TGATCATCAAATAATGATNACAGTGGCCA
   
Sequence Name 87-K101393-0022-833-C07C-IF40
GenBank Accession LN514742 [GenBank]
Sequencing primer gene specific primer, IF40: 5'-TATGTCATTGGCCACTGTTATCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K101393-0022-833-C07C-IF40
GATTTGCTCCTGCAAGATAGATCAGACATGCACAAGTTATGATAAAGACGAATGGATTCC
TTTTCGTACAACAAATTGTTTGGTCAAAACATGATGTTGCTTACTTCATTCTTATTTAAA
GGAAGATTCTGGGCTGTTTGAGGAGAGGCTTTGGATAAGTCCCTAAAAGCACCCTGAAAG
TATAAATGTCGTCCTCATTTAGACAGACTAATCATCGAATCACTAGTGCTACAAAATGAG
CAAGATAAAGAGAGTTGATCAAATTAAGAAGCAAGTATTGTCTTGCCTTGTTTGCCCACA
TAAGTCCACGTGGCTTCATGGATCAGCAATGAGTATCCTGAAGCAAGTATTGTCTTGCCT
TGTTTCCCGGACATGAAGCCATTTCCCGGACAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGGTCATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAAT

Last Updated on 06.12.2018 16:11