Sequences from confirmation amplicon


Confirmation sequences for line 836G11 and hit At5g02470 are shown.
Line ID 836G11
Locus At5g02470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:543349
Primers used for PCR JL23 - 8474, show primer details
   
Sequence Name 25-K032602-022-836-G11C-8409
GenBank Accession LN497331 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K032602-022-836-G11C-8409
AAAGGTGATCTTCTTTCTGTTTGATGGAAAATTTAAATACAAGACTCTCTTAGAATTTGA
GATCTTGTTATGCATGTCAATAGATGACAAAAGTTATTGATATTGCAGGTTTTCTAGAAT
CCATAGATACGTCGTGTATTGAATCATTTTATGTTGTCTTAATGAAATGTTAAACCGTCT
TTCTACGTTTTGCTTATAATGTTACTCAAATGTTGTTATCATCAGGTCTCAAGTCTTGAG
AGTCTTATGTCGAGAAATCAAGAGATGGTTGTGAAGACTCAAGGCCCAGCAGAAGGATTT
ACCTTACCATTCATTCTACTTGAGGTACTTCAAAAATCTCTTTCTCGTCCGTAGTTTCTA
TTTCATGTTTTTCGATATCCTAATCTATTTCCATCTCTATAGACAAACCCT
   
Sequence Name 25-K032602-022-836-G11C-JL23
GenBank Accession LN497332 [GenBank]
Sequencing primer gene specific primer, JL23: 5'-ACTACTGCGTGAGGGTTTGTCTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K032602-022-836-G11C-JL23
ATGAAAAGAAACTACGGACGAGAAAGAGATTTTTGAAGTACCTCAAGTAGAATGAATGGT
AAGGTAAATCCTTCTGCTGGGCCTTGAGTCTTCACAACCATCTCTTGATTTCTCGACATA
AGACTCTCAAGACTTGAGACCTGATGATAACAACATTTGAGTAACATTATAAGCAAAACG
TAGAAAGACGGTTTAACATTTCATTAAGACAACATAAAATGATTCAATACACGACGTATC
TATGGATTCTAGAAAACCTGCAATATCAATAACTTTTGTCATCTATTGACATGCATAACA
AGATCTCAAATTCTAAGAGAGTCTTGTATTTAAATTTTCCATCAAACAGAAAGAAGATCA
CCTTTTCTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACATTCACGCCCCAAAAGGGG
Primers used for PCR FP62 - 3144, show primer details
   
Sequence Name 02-K033039-022-836-G11C-FP62
GenBank Accession LN486924 [GenBank]
Sequencing primer gene specific primer, FP62: 5'-AAATCTACGTTTGAGACTGTGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K033039-022-836-G11C-FP62
GATTTTGCCACAATTAAGCAAAACGCAGAGAAGCCTTTGAATGAAAATGAGGTATAGCGA
GATGGAAAGCTCATATCATCTGTCTTGAGTTATTTGGAAAAAAAAAAAGCTATAACAACA
TTCTCTTATGTTCAATCAGTACAATGAGAAAAACATAAGGCGGAGAGTCTACAATGCGCT
CAATGTGTTCATGGCGTTGGATATTATTGCAAGGGATAAAAAGGAAATCCGGTGGAAAGG
ACTTCCTATTACCTGCAAAAAGGATGTGGAAAAAGTCAAGGTTTAGCTTCCAAGCTTTTC
CTTTCAGTTATCTCATCTATGTAATTGCTCTCTCTCTTCGTCTTCTCTACAAATCTCTCT
ACCTCTATTTGCAAATGGATCGTAATAAAGTTATGAGCAGTGTGCAAAAGAAGGCTGCTT
ATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAA
ATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTC
ATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAAACGTGGTTGGAAC
GTCTTCTTTTTCCACGATGCTCCTCGTGG
   
Sequence Name 34-K033039-022-836-G11C-3144
GenBank Accession LN501312 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K033039-022-836-G11C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATAAGCAGCCTTCTTTTGCAC
ACTGCTCATAACTTTATTACGATCCATCTGCAAATAGAGGTAGAGAGATTAGTAGAGAAG
ACGAAGAGAGAGAGCAATTACATAGATGAGATAACTGAAAGGAAAAGCTTGGAAGCTAAA
CCTTGACTTCTTCCACATCCTTTTTGCAGGTAATAGGAAGTCCTTTCCACCGGATTTCCT
TTTTATCCCTTGCAATAATATCCAACGCCATGAACACATTGAGCGCATCGTAGACTCTCC
GCCTTATGTTCTTCTCATTGTACTGATTGAACATAAGAGAATGTTGTTATAGCTTTTTTT
TTTTCCAAATAACTCAAGACAGATGATATGAGCTTTCCATCTCGCTATACCTCATTTTCA
TTCAAAGGCTTCTCTGCGTTTTGCTTAATTGGGGCAAAATCTGAAATAATTTCGTCTGCA
ACCTGGAATCACAAGAGCCACAGTC

Last Updated on 06.12.2018 16:11