Sequences from confirmation amplicon


Confirmation sequences for line 844C07 and hit At1g01720 are shown.
Line ID 844C07
Locus At1g01720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:269484
Primers used for PCR KY04 - 8474, show primer details
   
Sequence Name 09-K036056-022-844-C07C-8409
GenBank Accession LN490085 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K036056-022-844-C07C-8409
GCCATTTACATTGAATAATCCTGTAGCGGAAACATCTGATTACTACTCCCTCCTCCTCCA
AACGCGTTATCCACGGTGGCATCAATGTAATTAAACCCAAAATCAAGGGTATTGTTATTG
TCATTACTTACGGCCGACCAATCTTTCCACTTGGGCTCGCTCTGAACCTCGCTCGTGAAC
TCCGGCGACACCACCTGCTCCGAGCAACTCGAATCCGTAGTATGCAGCTTCGGCACCGAA
TCCGACGTGTCGAAATACGCGAACTCACTTGTCTGTTGCGGCGGCGGAGGCATAACCATC
TCCGTCACCTTCGGCTTCTCCTCCATGATTTCGTCGCCGTAAACAACCGGAGGCGGTGGT
CCCCGCCTCTCGGTAGCTCCTTTTTTGTTGTAAATCCGGCAGAGAACCCAATCATCCAGC
TGGGCAAAAATCAACACCGTTGATTCAA
   
Sequence Name 10-K036056-022-844-C07C-KY04
GenBank Accession LN490561 [GenBank]
Sequencing primer gene specific primer, KY04: 5'-TGTTTCTGGTAAATTGAATCAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K036056-022-844-C07C-KY04
GGTCCTCTGCCGGATTTACACAAAAAAGGAGCTACCGAGAGGCGGGGACCACCGCCTCCG
GTTGTTTACGGCGACGAAATCATGGAGGAGAAGCCGAAGGTGACGGAGATGGTTATGCCT
CCGCCGCCGCAACAGACAAGTGAGTTCGCGTATTTCGACACGTCGGATTCGGTGCCGAAG
CTGCATACTACGGATTCGAGTTGCTCGGAGCAGGTGGTGTCGCCGGAGTTCACGAGCGAG
GTTCAGAGCGAGCCCAAGTGGAAAGATTGGTCGGCCGTAAGTAATGACAATAACAATACC
CTTGATTTTGGGTTTAATTACATTGATGCCACCGTGGATAACGCGTTTGGAGGAGGAGGG
AGTAGTAATCAGATGTTTCCGCTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAA
ATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGCCCCAAA

Last Updated on 06.12.2018 16:11