Sequences from confirmation amplicon


Confirmation sequences for line 845F03 and hit At5g59670 are shown.
Line ID 845F03
Locus At5g59670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24042987
Primers used for PCR EU80 - 8474, show primer details
   
Sequence Name 35-K103787-0022-845-F03C-EU80
GenBank Accession LN501805 [GenBank]
Sequencing primer gene specific primer, EU80: 5'-AATGGAATTCAGAAAAATTGGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K103787-0022-845-F03C-EU80
GCTGAGATCCAGACTTGCAGGCAATGATACCAGAGAATTCACATCTTATTGAATGGACAA
AATTTATCTGTGACTGGCCCTGAAGTCCCAGATAAGCTAAGTATAAAGACCTTCCAAAGC
TCGTCACCGATAAGTTGCAATGGATGGGCATGTAATTTTCAGCTGATAAGAACCAAAAGA
TCAACTCTTCCACCTCTACTTAATGCTTTGGAAGTCTACACAGTTATTCAGCAATGAGTT
GAATAAATGGCTACACAGTCCCGGACATGAACTCTCTTCCCGGACAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCACAA
CGTTA
   
Sequence Name 51-K103788-0022-845-F03C-8409
GenBank Accession LN507481 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K103788-0022-845-F03C-8409
ATTTACAATTGAATATATCCTGTCCGGGAAGAGAGTTCATGTCCGGGACTGTGTAGCCAT
TTATTCAACTCATTGCTGAATAACTGTGTAGACTTCCAAAGCATTAAGTAGAGGTGGAAG
AGTTGATCTTTTGGTTCTTATCAGCTGAAAATTACATGCCCATCCNTTGCAACTTATCGG
TGACGAGCTTTGGAAGGTCTTTATACTTAGCTTATCTGGGACTTCAGGGCCAGTCACAGA
TAAATTTTGTCCATTCAATAAGATGTTGAATTCTCTGGTATCATTTGCCTGCAAGTCTTG
GATCTCAGCATAGTGTGCGTAAAAGTAATATTGGACATCCNATTTTTCTGAATTCCA

Last Updated on 06.12.2018 16:11