Sequences from confirmation amplicon


Confirmation sequences for line 846C09 and hit At4g17830 are shown.
Line ID 846C09
Locus At4g17830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9916812
Primers used for PCR 53tv - 8474, show primer details
   
Sequence Name 60-K117029-0022-846-C09C-53tv
GenBank Accession LN510354 [GenBank]
Sequencing primer gene specific primer, 53tv: 5'-AGCATAGATGGTGATAAGCTTCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K117029-0022-846-C09C-53tv
GGGCAAGCAAACCGGCGTCGAAAATCCAACGGTGGTGGCGGTTTTTCATCGCTAGCGAAG
AGAACTCTTCCATTCCTGGTGTTGGTGTGGACATTCCTGGTGAAAGACAAACTTCTTGAT
AAGCTCAAATCCGGACCCTTGTTAGTTTCCATTCTGTTAGAAATTTCTTGGGGAGTTTTT
AGATGAAAATTGAAGGATGATTGAAGCTTATTGATGTGTATTCTTTTGAAGGTATTGGAT
TGATACAGCGGGAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTC
   
Sequence Name 28-K117028-0022-846-C09C-8409
GenBank Accession LN499011 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K117028-0022-846-C09C-8409
ATTGAATATTCCCGCTGTATCAATCCAATACCTTCAAAAGAATACACATCAATAAGCTTC
AATCATCCTTCAATTTTCATCTAAAAACTCCCCAAGAAATTTCTAACAGAATGGAAACTA
ACAAGGGTCCGGATTTGAGCTTATCAAGAAGTTTGTCTTTCACCAGCATGTCCACACCAA
CACCAGGAATGGAAGAGTTCTCTTCGCTAGCGATGAAAACCGCCACCACCGTTGATTTCA
ACGCCGGTTTGGCTTGCCCGAGTTTCTTCATGAGTTCAGTGACAAGAGCAACGTGACCAA
GACAATCTGTTGTCCCACGACCACGAAGCTTATCACAATCTATGCTAGG

Last Updated on 06.12.2018 16:11