Sequences from confirmation amplicon


Confirmation sequences for line 847D03 and hit At2g25360 are shown.
Line ID 847D03
Locus At2g25360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10804329
Primers used for PCR KP28 - 8474, show primer details
   
Sequence Name 41-K030752-022-847-D03C-KP28
GenBank Accession LN503964 [GenBank]
Sequencing primer gene specific primer, KP28: 5'-AACGGCGACTAGATTCTACCATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K030752-022-847-D03C-KP28
GGCGAGGGTTGTTCTACGTCGTCCTATCCGACTCTGCTGTGAAGAAGTTTTCAGACAATG
TCGTCTACAGATTGTACACCAAGGGTTTGGTGGTGGAAGGTAAGGAAAAGACCGTGAAGT
CTGGATTAGAGTTGCGATTTGCGATGAGAAGGAGAATTTGTTGTATGAGATTAAAGAATC
ACTAAGCGACACTAAGATTAGCTGCATAGAAGTAGAGAGCATGGCACTGATTCGTGGGTT
AAGAGAATCCTTCGATTTGGGGATCAGACAAGTCGTGGTTTATTGCGATGACCAACTGCT
TCAAAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TTTCGACGTGTCTACATT
   
Sequence Name 02-K030752-022-847-D03C-8409
GenBank Accession LN486912 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K030752-022-847-D03C-8409
CATTTACAATTGAATATTTTTGAAGCAGTTGGTCATCGCAATAAACCACGACTTGTCTGA
TCCCCAAATCGAAGGATTCTCTTAACCCACGAATCAGTGCCATGCTCTCTACTTCTATGC
AGCTAATCTTAGTGTCGCTTAGTGATTCTTTAATCTCATACAACAAATTCTCCTTCTCAT
CGCAAATCGCAACTCTAATCCAGACTTCACGGTCTTTTCCTTACCTTCCACCACCAAACC
CTTGGTGTACAATCTGTAGACGACATTGTCTGAAAACTTCTTCACAGCAGAGTCGGATAG
GACGACTGTAGAACAACCCTCGCCTTTAGGGTTTGGGAAATCTCCGGTTATGGTAGAATC
TAGNTCGCC

Last Updated on 06.12.2018 16:11