Sequences from confirmation amplicon


Confirmation sequences for line 848H04 and hit At1g18320 are shown.
Line ID 848H04
Locus At1g18320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6304468
Primers used for PCR PF79 - 8474, show primer details
   
Sequence Name 07-K103223-0022-848-H04C-8409
GenBank Accession LN489229 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K103223-0022-848-H04C-8409
GCCTTTACATTGAATATGATGGTCAAGTTACAAGTTACATGTAGATGGCCATTTACAATT
CCCGGATGCCAACCATCGAGGAGATCCGAGCTCAAGAGGTTTGGAACAACTGCGCCGTTC
GTGCTGTAACTAGTGGAGTCATGGGTCAGTTCAAATATCCTCTCTTTCTCGATCTATATT
GCTCCATTGCTCTTGCTTTTCCAAAAATGCTAAAAGCTCTAGGTACTTTGATTGGTTTGA
GAATAGTGAAATTGGTAAAGTGATTAGCTTTCTAATGGTGATATGCTTCTGAGCTGGTTA
TTGAGTCACTTTCAGATTGCACTTAATAATCGGATTTGGTTAGGTGAATTGATGATGTGA
AATCTCAAATCGAATCTATTATTATAAATACTCTAAAAGGTATAAAGCTACTAATATAAA
TGCTCGCCGGTTGTAGAGTTGATATGACAGTTAGACATGTGAAGGATATTCAATTGATTT
GTTCACATTCTGTGTGAA
   
Sequence Name 55-K103230-0022-848-H04C-PF79
GenBank Accession LN508778 [GenBank]
Sequencing primer gene specific primer, PF79: 5'-TCACACAGAATGTGAACAAATCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K103230-0022-848-H04C-PF79
ATCAACTCTACAACCGGCGAGCATTTATATTAGTAGCTTTATACCCTTTAGAGTATTTAT
AATAATAGATTCGATTTGAGATTTCACATCATCAATTCACCTAACCAAATCCGATTATTA
AGTGCAATCTGAAAGTGACTCAATAACCAGCTCAGAAGCATATCACCATTAGAAAGCTAA
TCACTTTACCAATTTCACTATTCTCAAACCAATCAAAGTACCTAGAGCTTTTAGCATTTT
TGGAAAAGCAAGAGCAATGGAGCAATATAGATCGAGAAAGAGAGGATATTTGAACTGACC
CATGACTCCACTAGTTACAGCACGAACGGCGCAGTTGTTCCAAACCTCTTGAGCTCGGAT
CTCCTCGATGGTTGGCATCCGGGAATTGTAAATGGCCATCTACATGTAACTTGTAACTTG
ACCATCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TTTCGACGTGTCTACATTCACGTCCAAATG

Last Updated on 06.12.2018 16:11