Sequences from confirmation amplicon


Confirmation sequences for line 848H09 and hit At1g68200 are shown.
Line ID 848H09
Locus At1g68200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25562021
Primers used for PCR AK37 - 8474, show primer details
   
Sequence Name 04-K105708-0022-848-H09C-AK37
GenBank Accession LN487919 [GenBank]
Sequencing primer gene specific primer, AK37: 5'-TGATGAGACTGTTGAGCTCATTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K105708-0022-848-H09C-AK37
TTCTCTGACGAAGCGCTTCTGCTTCAGCGAAGCCTCATGGAGACGCGTGTAACACAACGC
CTCGCGATTCACCATATCTTGACGCTGTTGCATTATGTTGCGAGTCGACTTGTAGAGTTG
ATCAGAGTAGAGAGACGACGAAACGACGCCGCCACTGGATGAATTTCCGGCGGAGCTTCC
GCTGTAACTAAACGGCGCGATTTTGTTTTCCATTTTTCCCGGTGACAATTCTGTTACACA
AAATCGCCACAGAGAAATCAGAATTTATGCATCGGAAACGGAATCGCGAAGAATCAAACA
ACGGAAAATAAGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGGGT
TTATTA
   
Sequence Name 09-K105709-0022-848-H09C-8409
GenBank Accession LN490177 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K105709-0022-848-H09C-8409
TCGGACTATTTTCCGTTGTTGATTCTTCGCGATTCCGTTTCCGATGCATAAATTCTGATT
TCTCTGTGGCGATTTTGTGTAACAGAATTGTCACCGGGAAAAATGGAAAACAAAATCGCG
CCGTTTAGTTACAGCGGAAGCTCCGCCGGAAATTCATCCAGTGGCGGCGTCGTTTCGTCG
TCTCTCTACTCTGATCAACTCTACAAGTCGACTCGCAACATAATGCAACAGCGTCAAGAT
ATGGTGAATCGCGAGGCGTTGTGTTACACGCGTCTCCATGAGGCTTCGCTTGAAGCAGAA
GCGCTTCGTCTAGAGAACACTGAACTCCGATCGATGAATCTTCGTCTCAAGAATGAGCTC
ACANNTCTCATC

Last Updated on 06.12.2018 16:11