Sequences from confirmation amplicon


Confirmation sequences for line 854D01 and hit At2g46020 are shown.
Line ID 854D01
Locus At2g46020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18927723
Primers used for PCR DF17 - 8474, show primer details
   
Sequence Name 37-K029125-022-854-D01C-8409
GenBank Accession LN502495 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K029125-022-854-D01C-8409
GTAGATTTCCCGGACATGAAGCCATGCTGCCCATATTATTTATGTAATATTTGGTTTGAA
GTCAACGAAAGATAGCAAAAAAACTTATACGTATTCTAAAGAACACAGAAGAGTTCATCA
ACAGGATATGGCCATGTCAGCGCAACTAACAGCTAGTTAGCTAGAGTCAAAGTTAAGCTT
AGATAATGGATATAAGAGGCTTGTGGGTTCTCGCGGAACTGAGTGAGATAATTATGAGTT
GGGAGACTTGGGGTCTCAAATACTCCCTTGGTTCTAAGCATTGATTCTGTGGAAAATCAT
AAGAGAGTCTATCAGAAACACAAACCTTCCAATTCACCAGAACAGCATTGGGAACGATGA
TGAGATGCGGTCCATAATTCCCTTTAAACTCCATAAGGTAAGCAATCAGTGCCATTACCT
GTACAAAACAATTAGTATATTATGATAGGACAATATAATAATTTATTGGTCACCAAAGGC
GAAAGAAAAGTAAATCCAGGAATCCCTGCAAATCGGGAACAGATTTAACCTGAACAGTTT
TTCCAAGACCCATCTCATCAGCCAAGATCCCGTTTAATTTGTTATTATACAACGATAGCA
TCCATTGTAATCCAACCTGATTGGCACAGAGCAAAAAACCTTAGAA
   
Sequence Name 38-K029125-022-854-D01C-DF17
GenBank Accession LN502866 [GenBank]
Sequencing primer gene specific primer, DF17: 5'-CACGTTTTGATGGTTTCTAAGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K029125-022-854-D01C-DF17
TTTTGCTCTGTGCCAATCAGGTTGGATTACAATGGATGCTATCGTTGTATAATAACAAAT
TAAACGGGATCTTGGCTGATGAGATGGGTCTTGGAAAAACTGTTCAGGTTAAATCTGTTC
CCGATTTGCAGGGATTCCTGGATTTACTTTTCTTTCGCCTTTGGTGACCAATAAATTATT
ATATTGTCCTATCATAATATACTAATTGTTTTGTACAGGTAATGGCACTGATTGCTTACC
TTATGGAGTTTAAAGGGAATTATGGACCGCATCTCATCATCGTTCCCAATGCTGTTCTGG
TGAATTGGAAGGTTTGTGTTTCTGATAGACTCTCTTATGATTTTCCACAGAATCAATGCT
TAGAACCAAGGGAGTATTTGAGACCCCAAGTCTCCCAACTCATAATTATCTCACTCAGTT
CCGCGAGAACCCACAAGCCTCTTATATCCATTATCTAAGCTTAACTTTGACTCTAGCTAA
CTAGCTGTTAGTTGCGCTGACATGGCCATATCCTGTTGATGAACTCTTCTGTGTTCTTTA
GAATACGTATAAGTTTTTTTGCTATCTTTCGTTGACTTCAAACCAAATATTACATAAATA
ATATGGGCAGCATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATA

Last Updated on 06.12.2018 16:11