Sequences from confirmation amplicon


Confirmation sequences for line 854D06 and hit At5g13180 are shown.
Line ID 854D06
Locus At5g13180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4196394
Primers used for PCR DB28 - 8474, show primer details
   
Sequence Name 40-K030142-022-854-D06C-8409
GenBank Accession LN503596 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K030142-022-854-D06C-8409
GCCATTTACAATTGAATATTAAAAGAAAGAAAAGAAAAGATAGATTTGTGAAAATGGCGT
CAAAAGAAGAGAGGAAGGAGATTGAGTATGGTTTACGCCAAACGAAATACTAATAAATAA
TAATAAAGAAAAGGAGTTGTTCGTATCGTCTTCTACCTTATAAAACCCCACACCTTCCTT
GTTTCCCTCTTCCTACACCGCACTCTCTTCTTCTTCTTCTTCTTCCTCTCTGTTTTTTCT
CTCTCTTTGTTTGGAACCACCATGGATAATGTCAAACTTGTTAAGAATGGTGTTTTGAGA
TTGCCACCTGGATTCAGATTCCATCCTACTGATGAAGAACTTGTGGTTCAATACCTTAAG
AGGAAAGTTTGTTCTTCTCCTTTGCCAGCTTCAATCATCCCTGAGTTTGATGTTTGCAGA
GCTGATCCTTGGGATTTACCTGGTAAAGCTTTCTGATTCTTGCTATATTTAGTAAGTTTT
CAATTTAGAGTCTCTGTGACCTTTAAGGCTCATAAGATTCTCTTATGTTTTTTTAGGCA
   
Sequence Name 40-K030142-022-854-D06C-DB28
GenBank Accession LN503597 [GenBank]
Sequencing primer gene specific primer, DB28: 5'-TCTCTTTCTCCAAATTGCCTAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K030142-022-854-D06C-DB28
TATGAGCCTTAAGGTCACAGAGACTCTAAATTGAAAACTTACTAAATATAGCAAGAATCA
GAAAGCTTTACCAGGTAAATCCCAAGGATCAGCTCTGCAAACATCAAACTCAGGGATGAT
TGAAGCTGGCAAAGGAGAAGAACAAACTTTCCTCTTAAGGTATTGAACCACAAGTTCTTC
ATCAGTAGGATGGAATCTGAATCCAGGTGGCAATCTCAAAACACCATTCTTAACAAGTTT
GACATTATCCATGGTGGTTCCAAACAAAGAGAGAGAAAAAACAGAGAGGAAGAAGAAGAA
GAAGAAGAGAGTGCGGTGTAGGAAGAGGGAAACAAGGAAGGTGTGGGGTTTTATAAGGTA
GAAGACGATACGAACAACTCCTTTTCTTTATTATTATTTATTAGTATTTCGTTTGGCGTA
AACCATACTCAATCTCCTTCCTCTCTTCTTTTGACGCCATTTTCACAAATCTATCTTTTC
TTTTCTTTCTTTTAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACG
ACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGA
AATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11