Sequences from confirmation amplicon


Confirmation sequences for line 857H06 and hit At3g02130 are shown.
Line ID 857H06
Locus At3g02130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:382131
Primers used for PCR BB87 - 8474, show primer details
   
Sequence Name 60-K118446-0022-857-H06C-BB87
GenBank Accession LN510372 [GenBank]
Sequencing primer gene specific primer, BB87: 5'-ATATGTCAAAGCTGCCATCTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K118446-0022-857-H06C-BB87
TTCCGCTCAGATTAGAGCAACAGTGAAGCTAGATCCCCGAGACTTGTTGGGATTGGCCCG
AAGATCTGATTCACCGAGGCATCAAGAATCTTAAGAGAAGTGCACATGTTGTTTAGTCCC
TGAGGAATCCGACCTGAGAGCTTGTTGAAGCTTACATTGACATAAACAGCTTTGAGTTCG
TCGCAGTTATCAAACAGATTTCCCGGAAATTGCCCGTACAACCGATTCCCTCCAGCAGAA
AATATGTAAGAGACTCGCTTTCCCAACCGCTCCTGTGCAAGTGGTATAGACTTGAGAGTA
CCAGTGAAGTTGTTGTCTGCAAAATTATGAAACACGGCCGGACCTCCATCACTTCCAAGA
TCTATCAGGGAAGTTCCAACTTGAGCCTTCTCGGTAAAGAAGGAGAGATAAACAGACGAC
GGGTCGCTGTAAGATTCAATAGAAAACCTGTCAAAGTAAACAACAGGAGGACAATGGCTT
GTGGTATTGTTGAGAAAATCAGGGATTACACCCAAAGCTGTAAACAACAGGAGCTTCTCA
AAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGC
   
Sequence Name 20-K118445-0022-857-H06C-8409
GenBank Accession LN495421 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K118445-0022-857-H06C-8409
TCATTGAATATATCCTTTGAGAAGCTCCTGTTGTTTACAGCTTTGGGTGTAATCCCTGAT
TTTCTCAACAATACCACAAGCCATTGTCCTCCTGTTGTTTACTTTGACAGGTTTTCTATT
GAATCTTACAGCGACCCGTCGTCTGTTTATCTCTCCTTCTTTACCGAGAAGGCTCAAGTT
GGAACTTCCCTGATAGATCTTGGAAGTGATGGAGGTCCGGCCGTGTTTCATAATTTTGCA
GACAACAACTTCACTGGTACTCTCAAGTCTATACCACTTGCACAGGAGCGGTTGGGAAAG
CGAGTCTCTTACATATTTTCTGCTGGAGGGAATCGGTTGTACGGGCAATTTCCGGGAAAT
CTGTTTGATAACTGCGACGAACTCAAAGCTGTTTATGTCAATGTAAGCTTCAACAAGCTC
TCAGGTCGGATTCCTCAGGGACTAAACAACATGTGCACTTCTCTTAAGATTCTTGATGCC
TCGGTGAATCAGATCTTCGGGCCAATCCCAACAAGTCTCGGGGATCTAGCTTCACTTGTT
GCTCTTAATCTGAGCTGGAATCAGTTGCAAGGTCAGATACCGGGTAGCCTCGGGAAGAAG
ATGGCAGCTTTTGACAT
Primers used for PCR EU84 - 8474, show primer details
   
Sequence Name 03-K103845-0022-857-H06C-EU84
GenBank Accession LN487459 [GenBank]
Sequencing primer gene specific primer, EU84: 5'-AGCTTGAGGTTTTGGATGTTTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K103845-0022-857-H06C-EU84
TGTTGACTTGGAATTGCTCTTCGTTGTCTGTTCTCGTTCTATCAAATCTGTACAATGTGT
ATGAAGACATTAATAGTGTAAGAGGGGAAGCTGATCTTCCTCCAGGCGCTGATTTAACCT
CGATGACCGAGGATTTCAATTTCTATCAAGGAGGAATCCCGGAGGAAATCACTAGGCTTC
CGAAACTAAAGATACTTTGGGTGCCCAGAGCTACGTTAGAAGGTAGATTTCCAGGAGATT
GGGGTTCATGTCAAAACTTGGAGATGGTTAATTTGGGTCAGAACTTCTTTAAAGGCGAAA
TTCCTGTCGGTCTTAGTAAATGCAAGAACCTTCGTCTTCTTGATTTGAGCTCGAATAGGC
TTACTGGGGAGCTTCTCAAAGAGATTTCGGTCCCATGCATGAGTGTTTTTGATGTTGGTG
GAAACAGCTTATCGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCANAAATG
   
Sequence Name 19-K103846-0022-857-H06C-8409
GenBank Accession LN494731 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K103846-0022-857-H06C-8409
ATCCGGAATGAGCCATTTACATTGAATATATCCGATAAGCTGTTTCCCCAACATCAAAAA
CACTCATGCATGGGACCGAAATCTCTTTGAGAAGCTCCCCAGTAAGCCTATTCGAGCTCA
AATCAAGAAGACGAAGGTTCTTGCATTTACTAAGACCGACAGGAATTTCGCCTTTAAAGA
AGTTCTGACCCAAATTAACCATCTCCAAGTTTTGACATGAACCCCAATCTCCTGGAAATC
TACCTTCTAACGTAGCTCTGGGCACCCAAAGTATCTTTAGTTTCGGAAGCCTAGTGATTT
CCTCCGGGATTCCTCCTTGATAGAAATTGAAATCCTCGGTCATCGAGGTTAAATCAGCGC
CTGGAGGAAGATCAGCTTCCCCTCTTACACTATTAATGTCTTCATACACATTGTACAGAT
TTGATAGAACGAGAACAGACNACGAAGAGCAATTTCCAAGTTCAACAGGTAATGGACCAC
TAAGAGTGTTCCTAGAAACATC

Last Updated on 06.12.2018 16:11