Sequences from confirmation amplicon


Confirmation sequences for line 859H07 and hit At2g29680 are shown.
Line ID 859H07
Locus At2g29680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12690784
Primers used for PCR HV52 - 8474, show primer details
   
Sequence Name 91-K101387-0022-859-H07C-8409
GenBank Accession LN515106 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K101387-0022-859-H07C-8409
GCCATTTACAATTGAATATATCCTGTAAATGGCTACATGTAGATTTTACATGTAGATTTT
CATGAAGGACACCTCGGTCTCTTGTGATCAAGTAATCCATTTCATCTGCAATTATTAGCC
TGCAGATAAAGTTTGAATGAGAAACAGCGCAACAAATTATATCTACTTATCTAGCAGGTC
ATCATATCACAAGACCAAAAACAAAAGAAAAACCTACATCATCTTTGATCTGGATTGTTG
TTGCTTTTGAGAAAACAATCTCTGAAGTTGTTGTAGAGGTGAAAATGAACCATTAGCTTT
CTTCCCGGACTCATAGTTACCAAGTATCTAAAAACAAATAAACTCATTAACAACTATACT
GAAATGA
   
Sequence Name 91-K101393-0022-859-H07C-HV52
GenBank Accession LN515107 [GenBank]
Sequencing primer gene specific primer, HV52: 5'-ACCAATCTTTGTGCATGTTTCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K101393-0022-859-H07C-HV52
ATCTTTCTGAATTCTTACAGGCAGGTTTGCATTGTCCTGAAACAGTGTCTGTTAATTGCA
CATCACTGACAAAAAGTACAGATATTTTCTCCAAGGTAAGACCTTATCCAGTCGAAAATT
ACTCGAAGTTATTGGTCTCATCATTTCAGTATAGTTGTTAATTGAGTTATTTTGTTTTTA
GATACTTGGTAACTATGAGTCCGGGAAGAAAGCTAATGGTTCATTTTCACCTCTACAACA
ACTTCAGAGATTGTTTTCTCAAAAGCAACAACAATCCAGATCAAAGATGATGTAGGTTTT
TCTTTTGTTTTTGGTCTTGTGATATGATGACCTGCTAGATNAGTAGATATAATTTGTTGC
GCTGTTTCTCATTCAAACTTTATCTGCAGGCTAATAATTGCAGATGAAATGGATTACTTG
ATCA

Last Updated on 06.12.2018 16:11