Sequences from confirmation amplicon


Confirmation sequences for line 862B04 and hit At1g74060 are shown.
Line ID 862B04
Locus At1g74060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27851366
Primers used for PCR CN73 - 8474, show primer details
   
Sequence Name 25-K104153-0022-862-B04C-CN73
GenBank Accession LN497416 [GenBank]
Sequencing primer gene specific primer, CN73: 5'-AAAAATTTCGGGAGATTCAAAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K104153-0022-862-B04C-CN73
TCCTTAGACCAAGAAACAACTATTGCATTGAGCTAGATTTCAGACTCCAAGACAATGAGA
CAGTGTATCAGCTAAGTACCTGAGCTTGGCTGGTTTTGCCGTGCGCCTGTTGGGGAGAGG
TTTCTTCACGTCTTCAGCTGGATAGAACTTCGGTGGCTTCTCCACCGGAGCATCAACCTT
AGATTTAGCGTCGTGGCGGGGGAAAACGCCTCCATTTTTGGCCTTGATTGCCCACAAACC
TCTCTTATGGTACATCTGAGACCTTGAGTATTTACCAACTCCCCTGATCAGATCAGGATT
CCTGTTGACCTTAGCTGTCCTCTGTTTCGCCGGCATCGTTACTTTCTCTCTGTTGCAATC
CCTGGACGATAATCAAGAAGAGACTTTCCGGCCGAGAATAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 25-K104154-0022-862-B04C-8409
GenBank Accession LN497417 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K104154-0022-862-B04C-8409
GCCATTTATTCTCGGCCGGAAAGTCTCTTCTTGATTATCGTCCAGGGATTGCAACAGAGA
GAAAGTAACGATGCCGGCGAAACAGAGGACAGCTAAGGTCAACAGGAATCCTGATCTGAT
CAGGGGAGTTGGTAAATACTCAAGGTCTCAGATGTACCATAAGAGAGGTTTGTGGGCAAT
CAAGGCCAAAAATGGAGGCGTTTTCCCCCGCCACGACGCTAAATCTAAGGTTGATGCTCC
GGTGGAGAAGCCACCGAAGTTCTATCCAGCTGAAGACGTGAAGAAACCTCTCCCCAACAG
GCGCACGGCAAAACCAGCCAAGCTCAGGTACTTAGCTGATACACTGTCTCATTGTCTTGG
AGTCTGAAATCTAGCTCAATGCAATAGTTGTTTTCTTTGGTTCTTAAGGAGTGCTTTGTA
TGCATTTAAATCTTTGAATCTCCGAAAATTTT

Last Updated on 06.12.2018 16:11