Sequences from confirmation amplicon


Confirmation sequences for line 862G11 and hit At4g13250 are shown.
Line ID 862G11
Locus At4g13250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7686938
Primers used for PCR NA54 - 8474, show primer details
   
Sequence Name 23-K101563-0022-862-G11C-8409
GenBank Accession LN496493 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K101563-0022-862-G11C-8409
CTTTACAATTGAATATATCCTGTAACGTGTGGCCATCACACTTCTCTTCCTTCTTTCTTT
CCCTCACCTCTTTCTTTCTTCGTCCTAATCTCCTTCTTCTTCCTCCGATCACTCTATTTA
ACGTCTTCGACGAATCCGAAGCTGAAAAATATCTAACAATCTATCTTTTGGGTCCCTAAA
AAAGCTCCTTCGTTTATCTTGTTTTCTAAGTTATCTTTTTGTATGAGTTTCAAAGCTTAT
CATTATCTCACTCTTCTTCCATAGCTATGACTACTTTAACGAAGATTCAAGTGTACCCAC
AAGTCTTGGAGCACCGTCTCTTCTTCAGAGATCCGATACGGGTCGGGTCAAGATTGACTT
GTAGAGAACGTAGTAATAGGGTTTATGTGCATCGG
   
Sequence Name 23-K101576-0022-862-G11C-NA54
GenBank Accession LN496494 [GenBank]
Sequencing primer gene specific primer, NA54: 5'-CCGATGCACATAAACCCTATTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K101576-0022-862-G11C-NA54
GACCGTATCGGATCTCTGAAGAAAGACGGTGCTCCAAGACTTGTGGGTACACTTGAATCT
TCGTTAAAGTAGTCATAGCTATGGAAGAAGAGTGAGATAATGATAAGCTTTGAAACTCAT
ACAAAAAGATAACTTAGAAAACAAGATAAACGAAGGAGCTTTTTTAGGGACCCAAAAGAT
AGATTGTTAGATATTTTTCAGCTTCGGATTCGTCGAAGACGTTAAATAGAGTGATCGGAG
GAAGAAGAAGGAGATTAGGACGAAGAAAGAAAGAGGTGAGGGAAAGAAAGAAGGAAGAGA
AGTGTGATGGCCACACGTTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT

Last Updated on 06.12.2018 16:11