Sequences from confirmation amplicon


Confirmation sequences for line 862G11 and hit At5g67420 are shown.
Line ID 862G11
Locus At5g67420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26905587
Primers used for PCR 03cy - 8474, show primer details
   
Sequence Name 18-K109234-0022-862-G11C-03cy
GenBank Accession LN494342 [GenBank]
Sequencing primer gene specific primer, 03cy: 5'-GAGAGATAGATCAAGCTCCGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K109234-0022-862-G11C-03cy
GATATCTCTACGTTTCTCGGAGAAGAAGAATCCATAGTAGATCTAGATCTAGAGCTTGAG
AATCGTGAATGATGATAGATGTTACGATCGGTGGAATCATTTTGCTGGAGATTCAACATT
TCGGTGCAGATCTCAGATGCTTCTTCAGATGTAGGCGAAGGAAAGCCAGCGAAACCGCCG
CCGTGAGTGAGAAGCTCCGGGATCGGTCTTAAAGAACCGCCGCGAAGCACTGTTTCAACA
GCCGCTTGACAGATATTCCAGTTTCCAGTCCATAACATTCCGATTGCTCCGTTAACTGGA
TTGACAGTTCTTCCACAAGCTTCGTAGAGCAACGACTGAAACAAAGCTGCACCACAAAAA
CCGGAACAAAGATGAGAAAAACGAACGATGAAACTGAAAATCCGGTTTAAAGATCTGGTT
TAGTAGTAAGTACCAGGACGTTGAGAATCCGGAACAGCGGAGATAAAGGACATGAGACCA
GCACGGCCGAAGAATTTAGCGACGAAGACGGTGGCGTGGCCTTGAGCATCGGCGGTTTCA
ATCCATTGAATACATGGCCGGAGGATACAATTCTCGCTGCAACCTTTCCGGAGAACACGG
CAACCATTGCAGCTCATCTTTTCAGATTCAAGTTTTAAGTTATTTCCTCCGGTGAATTTG
GAGTTTGTATTGTTTTAATTTAAGGTTTTAATCTCCTGAATCTTGCTGATGTCTGGGAGA
AGAAGAAGAAGAAAGTGATATCTTTGCNCNGACNAAAAAAAGAGAAGAGAGATTAAAATG
TTTTATGAATATTTTCAATTGTAAATGGCTTCATGTCCGGGGAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCATTCA
AAAATGT
   
Sequence Name 02-K109233-0022-862-G11C-8409
GenBank Accession LN487070 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K109233-0022-862-G11C-8409
CATTTACAATTGAAAATATTCATAAAACATTTTAATCTCTCTTCTCTTTTTTTTGTCTGT
GCAAAGATATCACTTTCTTCTTCTTCTTCTCCCAGACATCAGCAAGATTCAGGAGATTAA
AACCTTAAATTAAAACAATACAAACTCCAAATTCACCGGAGGAAATAACTTAAAACTTGA
ATCTGAAAAGATGAGCTGCAATGGTTGCCGTGTTCTCCGGAAAGGTTGCAGCGAGAATTG
TATCCTCCGGCCATGTATTCAATGGATTGAAACCGCCGATGCTCAAGGCCACGCCACCGT
CTTCGTCGCTAAATTCTTCGGCCGTGCTGGTCTCATGTCCTTTATCTCCGCTGTTCCGGA
TTCTCAACGTCCTGGTACTTACTACTAAACCAGATCTTTAAACCGGATTTTCAGTTTCAT
CGTTCGTTTTTCTCATCTTTGTTCCGGTTTTTGTGGTGCAGCTTTGTTTCAGTCGTTGCT
CTACGAAGCTTGTGGAAGAACTGTCAATCCAGTTAACGGAGCAATCGGAATGTTATGGAC
TGGAAACTGGAATATCTGTCAAGCGGCTGTTGAAACAGTGCTTCGCGGCGGTTCTTTAAG
ACCGATCCCGGAGCTTCTCACTCACGGCGGCGGTTTCGCTGGCTTTCCTTCGCCTACATC
TGAAGAAGCATCTGAGATCTGCACCGAAATGTTGAATCTCCAGCAAAATGATTCCACCGA
TCGTAACATCTATCATCATTCACGATTCTCAAGCTCTAGATCTAGATCTACTATGGATTC
TTCTTCTCCGACGAAACGTAAGAGATTATCATCGGAAGACCAACCATCTTCGGA

Last Updated on 06.12.2018 16:11