Sequences from confirmation amplicon


Confirmation sequences for line 863D08 and hit At4g38160 are shown.
Line ID 863D08
Locus At4g38160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17902307
Primers used for PCR DL04 - 8474, show primer details
   
Sequence Name 81-K027491-022-863-D08C-8409
GenBank Accession LN514005 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K027491-022-863-D08C-8409
CCATTTACATTGAATATAGCCGGAAAGGCTCTCCTCCAAGGTAAATTCCTCTCTACTTCC
TTGTTAAAGCTCGAATCATCTCTAAGTTAAGCAAAATTGGTGTGTTGTAGTGGCGTTTAT
ATGGAGGTGACAAATACGAGCAGCATCATGTGGTTCTTCAGGGACAAAGGTTTCGATGAT
CCGAGTATCGACAAGATGTTGAGAAAATGCAAACAGTTGGAAAAGGCTCAAAGCGATGTG
GCATCAGAAAACTGGGACTACCTGAGTAACATTGTTGGTATCCAAGAGAGAAAACTCCCT
TACATCGTCTCTCGATGCCCCAAAATCCTGACTTTACGCCTCGATGAGAGACTCATCCCG
ATGGTCGAGTGCCTCTCCAGTCTTGGAAGGAATCCTCGGGAAGTTGCTTCCGCCATTACC
AAATTTCCTCCAATACTCTCTC
   
Sequence Name 82-K027491-022-863-D08C-DL04
GenBank Accession LN514128 [GenBank]
Sequencing primer gene specific primer, DL04: 5'-TCCTCCACGCTATGAGAGAGTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K027491-022-863-D08C-dl4
GCGGAGCACTTCCGAGGATTCCTTCCAGACTGGAGAGGCACTCGACCATCGGGATGAGTC
TCTCATCGAGGCGTAAAGTCAGGATTTTGGGGCATCGAGAGACGATGTAAGGGAGTTTTC
TCTCTTGGATACCAACAATGTTACTCAGGTAGTCCCAGTTTTCTGATGCCACATCGCTTT
GAGCCTTTTCCAACTGTTTGCATTTTCTCAACATCTTGTCGATACTCGGATCATCGAAAC
CTTTGTCCCTGAAGAACCACATGATGCTGCTCGTATTTGTCACCTCCATATAAACGCCAC
TACAACACACCAATTTTGCTTAACTTAGAGATGATTCGAGCTTTAACAAGGAAGTAGAGA
GGAATTTACCTTGGAGGAGAGCCTTTCCGGCTATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11