Sequences from confirmation amplicon


Confirmation sequences for line 863H12 and hit At2g24740 are shown.
Line ID 863H12
Locus At2g24740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10531964
Primers used for PCR HP57 - 8474, show primer details
   
Sequence Name 27-K033706-022-863-H12C-8409
GenBank Accession LN498281 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K033706-022-863-H12C-8409
TCGGACTGAGCCTTTACAGTGAAAACCCACCTTATTTCCCCTCATACAGTAATGGTCTCA
ACACCTCCAACCCTATTAATGCTATTTGATGATGGAGATGCTGGTCCTAGTACTGGTTTA
GTCCACCGAGAAAAATCTGATGCTGTTAATGAAGAAGCCCACGCAACATCAGTACCTCCT
CATGCACCGCCACAAACCCTTTGGCTATTAGATAACTTCAACATTGAGGACTCTTATGAC
CGAGATGCTGGTCCTAGTACTGGTCCAGTCCACAGAGAAAGATCAGATGCTGTTAATGAA
GAAGCCCACGCAACATCAATACCTCCTCATGCACCGCCACAAACCCTTTGGCTATTAGAT
AACTTCAACATTGAGGACTCTTATGACCGAGATGCCGGTCCTAGTACTAGTCCAATAGAC
CGAGAAGCATCTCATGAAGTCAATGAAGACGCCCACGCAACATCAGCACCTCCTCATGTA
ATGGTCTCACCACTACAAAACAGAAGACCATTTGATCAATTCAACAACCAACCTTATGAT
GCGAGTGCTGGTCCTAGTACTGGTCCAGGAAAGCGAGGACGTGGCCGACCAAAGGGTTCG
AAAAATGGGTCGAGAAAGCCAAAAAAGCCAAAAGCATATGACAACAACTCTACTGATGCG
AGTGCTGGTCCTAGTTCTGGTCTAGGA
   
Sequence Name 27-K033706-022-863-H12C-HP57
GenBank Accession LN498282 [GenBank]
Sequencing primer gene specific primer, HP57: 5'-ACATCTTCGTTTTCCTAGACCAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K033706-022-863-H12C-HP57
GTTGTTGTCATATGCTTTTGGCTTTTTTGGCTTTCTCGACCCATTTTTCGAACCCTTTGG
TCGGCCACGTCCTCGCTTTCCTGGACCAGTACTAGGACCAGCACTCGCATCATAAGGTTG
GTTGTTGAATTGATCAAATGGTCTTCTGTTTTGTAGTGGTGAGACCATTACATGAGGAGG
TGCTGATGTTGCGTGGGCGTCTTCATTGACTTCATGAGATGCTTCTCGGTCTATTGGACT
AGTACTAGGACCGGCATCTCGGTCATAAGAGTCCTCAATGTTGAAGTTATCTAATAGCCA
AAGGGTTTGTGGCGGTGCATGAGGAGGTATTGATGTTGCGTGGGCTTCTTCATTAACAGC
ATCTGATCTTTCTCTGTGGACTGGACCAGTACTAGGACCAGCATCTCGGTCATAAGAGTC
CTCAATGTTGAAGTTATCTAATAGCCAAAGGGTTTGTGGCGGTGCATGAGGAGGTACTGA
TGTTGCGTGGGCTTCTTCATTAACAGCATCAGATTTTTCTCGGTGGACTAAACCAGTACT
AGGACCAGCATCTCCATCATCAAATAGCATTAATAGGGTTGGAGGTGTTGAGACCATTAC
TGTATGAGGTGGAAATAAGGTGGGTTTTCACTTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATNTCGACGTGTCTACATC

Last Updated on 06.12.2018 16:11