Sequences from confirmation amplicon


Confirmation sequences for line 872B07 and hit At2g15370 are shown.
Line ID 872B07
Locus At2g15370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:6699891
Primers used for PCR KQ90 - 8474, show primer details
   
Sequence Name 06-K101499-0022-872-B07C-8409
GenBank Accession LN488744 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K101499-0022-872-B07C-8409
TAAGTATTCAAAGTCTATTTAACTGATCTATGATTGACACATAGAAATGAAGCTATATAA
AAGTTGTACTCTCTTTTTGAACCATCTCACAATCAAACTCAAGTCAACATGTATCAAAAA
TTTCAGATCTCCGGCAAAATTGTTAAGACTTTGGGGCTAAAGATGAAAGTTCTGATAGCA
GTCTCCTTTGGTTCCTTACTATTTATACTATCATACTCAAACAACTTTAACAACAAACTT
CTTGATGCTACAACCAAAGGTAAGAAAATTATCCATATCTGGTGTTTTATTGTTAAGTCA
ATGAATCCTCATTTTGGTT
   
Sequence Name 06-K101503-0022-872-B07C-KQ90
GenBank Accession LN488745 [GenBank]
Sequencing primer gene specific primer, KQ90: 5'-AAACCAAAATGAGGATTCATTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K101503-0022-872-B07C-KQ90
TGGATATTTTCTTACCTTTGGTTGTAGCATCAAGAAGTTTGTTGTTAAAGTTGTTTGAGT
ATGATAGTATAAATAGTAAGGAACCAAAGGAGACTGCTATCAGAACTTTCATCTTTAGCC
CCAAAGTCTTAACAATTTTGCCGGAGATCTGAAATTTTTGATACATGTTGACTTGAGTTT
GATTGTGAGATGGTTCAAAAAGAGAGTACAACTTTTATATAGCTTCATTTCTATGTGTCA
ATCATAGATCAGTTAAATAGACTTTTGAATACTTTAATGCGTCATTGTTAATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CANAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11