Sequences from confirmation amplicon


Confirmation sequences for line 873G07 and hit At5g17420 are shown.
Line ID 873G07
Locus At5g17420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5737260
Primers used for PCR NK57 - 8474, show primer details
   
Sequence Name 44-K103393-0022-873-G07C-NK57
GenBank Accession LN505151 [GenBank]
Sequencing primer gene specific primer, NK57: 5'-TTAGGCTTTTGTTGTCGTTTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K103393-0022-873-G07C-NK57
TTAGAACAGTCTAGGCTCTCTGTTAAAATTTGCATTCAAGATTTGTGGGTTTTCACTGCA
ACTACATGTCTCTTGAGGGAAGTATCTGTGAATCTTTTACTGTGTAACTAAAAAGTAGTT
TTGTTCAAATTTTCTTACAATGCATTACTCGCTCACAATAAACCCAGATCCATCTGTTTT
ACTGGCTAATATACAATAAACAAAATCCAATTATGCAATCCAAAGAGAGAAGTGAAAACA
CTCTCGACAAAGTACAGAAAAAGTTTCAGAAGACGAAAAAGATTTTGCTTCAGCAGTTGA
TGCCACACTTGGAAGTGTCAGGTCCTTTGGTCTTGAGCACAAAAGGATCAATTCTTACCC
AAAGCAAAGAGAAGATAGATGCCAATAACACTGACCAAATCACCACAATGGTTGGTGTTC
TGTTCTGTCTACCCATCAGACCTTTGAGGAATGGGTAGAGATGAACAATGACCCAAAAGG
AGAAGAAGAGTTTACCAAATAGAGGTCCCCAAGACTGATATCCATTGTTAATGGCATCTG
AGATGCCTGCAACAACGCCNACAATGTTTATGATTAAGACAGTTGTTGGAGGGATCAGCA
GTGTTGTCCATTTGAATGCGTAAAGTTCTCCAAAGTCATCATCATCTGTTGCCTTTGATG
TGACGGTGAAGTTTGTGTCAATGCCTGCTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAG
   
Sequence Name 34-K103394-0022-873-G07C-8409
GenBank Accession LN501380 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K103394-0022-873-G07C-8409
CCATTTAGCAGGCATTGACACAAACTTCACCGTCACATCAAAGGCAACAGATGATGATGA
CTTTGGAGAACTTTACGCATTCAAATGGACAACACTGCTGATCCCTCCAACAACTGTCTT
AATCATAAACATTGTTGGCGTTGTTGCAGGCATCTCAGATGCCATTAACAATGGATATCA
GTCTTGGGGACCTCTATTTGGTAAACTCTTCTTCTCCTTTTGGGTCATTGTTCATCTCTA
CCCATTCCTCAAAGGTCTGATGGGTAGACAGAACAGAACACCAACCATTGTGGTGATTTG
GTCAGTGTTATTGGCATCTATCTTCTCTTTGCTTTGGGTAAGAATTGATCCTTTTGTGCT
CAAGACCAAAGGACCTGACACTTCCAAGTGTGGCATCAACTGCTGAAGCAAAATCTTTTT
CGTCTTCTGAAACTTTTTCTGTACTTTGTCGAGAGTGTTTTCACTTCTCTCTTTGGATTG
CATAATTGGATTTTGTTTATTGTATATTAGCCAGTAAAACAGATGGATCTGGGTTTATTG
TGAGCGAGTAATGCATTGTAAGAAAATTTGAACAAAACTACTTTTTAGTTACACAGTAAA
AGATTCACAGATACTTCCCTCAAGAGACATGTAGTTGCAGTGAAAACCCACAAATCTNGA
ATGCAAATTTTTAACAGAGAGCCTGAGACTTGTTCTTATATCGTGGAGTTCACAAACAAA
ATAAAGAAACGACCACAAAAGCCT

Last Updated on 06.12.2018 16:11