Sequences from confirmation amplicon


Confirmation sequences for line 879D07 and hit At5g07370 are shown.
Line ID 879D07
Locus At5g07370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2331790
Primers used for PCR JL24 - 8474, show primer details
   
Sequence Name 59-K032524-022-879-D07C-8409
GenBank Accession LN509902 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K032524-022-879-D07C-8409
CATTTACAATTGAATATATCCTGTCCGGGAAATTCAATTGTAAGATGATACAAACTTCCT
CAGAGTCAATCTCGCTCCATCTACATCGAGCCCGCGAAGAAGCTTCCTCTCGGGCTTCCA
GAAACTCGATTCTTTGTGATCATACACTTCGAAACCAGAGATCCTGAAACCCGATGACAC
GGTTGTGGTACCCGTGTCTTTCTTCAAACATTTTTGGATGTATTCTTCAGATGCATCAGG
ATACCATGTTCTCGAACCCATCTTAACATCCATTACTGATGGTTTTGTGTATTCTGCAAG
AAGATTTTCCAACACCATCATGGCTGCTCCATCAGAACCTTCAACTGCTTGAGTGCCGTG
ATACACCGGGAAATATCTATGGATGTGTTCTGGAACCTCTGTGTTTGAGGGAGAAA
   
Sequence Name 59-K032524-022-879-D07C-JL24
GenBank Accession LN509903 [GenBank]
Sequencing primer gene specific primer, JL24: 5'-CTTTCTCCTCAAACACAGAGGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K032524-022-879-D07C-JL24
GGCACTCAAGCAGTTGAAGGTTCTGATGGAGCAGCCATGATGGTGTTGGAAAATCTTCTT
GCAGAATACACAAAACCATCAGTAATGGATGTTAAGATGGGTTCGAGAACATGGTATCCT
GATGCATCTGAAGAATACATCCAAAAATGTTTGAAGAAAGACACGGGTACCACAACCGTG
TCATCGGGTTTCAGGATCTCTGGTTTCGAAGTGTATGATCACAAAGAATCGAGTTTCTGG
AAGCCCGAGAGGAAGCTTCTTCGCGGGCTCGATGTAGATGGAGCGAGATTGACTCTGAGG
AAGTTTGTATCATCTTACAATTGAATTTCCCGGACAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
Primers used for PCR FP61 - 8474, show primer details
   
Sequence Name 74-K118446-0022-879-D07C-FP61
GenBank Accession LN513149 [GenBank]
Sequencing primer gene specific primer, FP61: 5'-AATATTTGGGATCTCACTGCAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K118446-0022-879-D07C-FP61
GTGTGATTATTTCCTCTTTTGGCGCTAAACTTTTTTAGCTGTACAAGTCACATTCAAAGT
AAAGTTCAAATATGGAATTTTTTTATATGCCTAGAAATGGAATACTCTTTGAGAAAAGAG
TACGAATGTATTCTGCTCTTTAGGTAATCAAGTACATATGTGAAGGAAACATAAGTTTAT
ATCCAAAAATTGGAATCAGCCAAAGCCATAACAAACAAATTAACTTAAATCTAAGAATCT
GCAGACTCATCTGGGCTCTGAAGAATCTCACGAATGAAGTTTATGAAAGAGCAAAGACCA
CCCAAGAAGTTATGGTCAATGACACCATTACCATCAAGAACATGAGCAAAATCCACCAGC
TTGACTTGTGGTCTAGCATCATCATCATTTCCCTTTAAGATGGATTCATTCTCATAGACC
ATTAAAATCGAACAAGAGTTGAAATGGTAGAGCGTTTGGTTCTCGAACCAGGTCTTGAGT
TCCAGCAACTGCGTTAAGATCCCGTGGGAACCGCCGTAAACACTCGAGGCAAAAGCAGAG
TCAGGTTTCGAGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 34-K118445-0022-879-D07C-8409
GenBank Accession LN501598 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K118445-0022-879-D07C-8409
CATTTCATTGAATATATCCTGACTCGAAACCTGACTCTGCTTTTGCCTCGAGTGTTTACG
GCGGTTCCCACGGGATCTTAACGCAGTTGCTGGAACTCAAGACCTGGTTCGAGAACCAAA
CGCTCTACCATTTCAACTCTTGTTCGATTTTAATGGTCTATGAGAATGAATCCATCTTAA
AGGGAAATGATGATGATGCTAGACCACAAGTCAAGCTGGTGGATTTTGCTCATGTTCTTG
ATGGTAATGGTGTCATTGACCATAACTTCTTGGGTGGTCTTTGCTCTTTCATAAACTTCA
TTCGTGAGATTCTTCAGAGCCCAGATGAGTCTGCAGATTCTTAGATTTAAGTTAATTTGT
TTGTTATGGCTTTGGCTGATTCCAATTTTTGGATATAAACTTATGTTTCCTTCACATATG
TACTTGATTACCTAAAGAGCAGAATACATTCGTACTCTTTTCTCAAAGAGTATTCCATTT
CTAGGCATATAAAAAAATTCCATATTTGAACTTTACTTTGAATGTGACTTGTACAGCTAA
AAAAGTTTAGCGCCAAAAGAGGAAATAATTCACACTAATAGTAAATTTCGTAAATAATTA
AGAGTTTGTTTGCAGTGAGACCCCCAAATA

Last Updated on 06.12.2018 16:11