Sequences from confirmation amplicon


Confirmation sequences for line 902G09 and hit At1g80180 are shown.
Line ID 902G09
Locus At1g80180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30157561
Primers used for PCR KF29 - 8474, show primer details
   
Sequence Name 67-K103357-0022-902-G09C-KF29
GenBank Accession LN511793 [GenBank]
Sequencing primer gene specific primer, KF29: 5'-GATCAGTCTGCAACAAAAACAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K103357-0022-902-G09C-KF29
AACAGTTTAACATTAAAGCACAAACAAAAAAACACAAACAAAAACAGAAAGAAAAAGATT
CATAAGAAGCAGTATAAGTATATATACTCTCTTTTTGTTTTGTTTTGTTTTCTCATCTTT
GTGTGTGTTCTTCTTCTTTACTTTAGACTTTAGTTCATGCCTATCTGGATCTACGCTTCG
CTGGCCTTTTCCTCTGATTAACCTTCTTCCCCGGCTGTTTCTTCCCGAAGGCAGAGCAGC
ACCCAAACGCCGACAGTCTCGGCGACGGCGGGTCCACCGCCGGAGACACTCTCCCTGTGT
TTCGATGGTGGCGGATGGCTCCTCCTCCGTTGGATCGACTCCTCTCCATACCTCCGTCAG
TTCTGATAGGTTTGAGTCCTTCTCCGGCGATAGGTTTGGTTGTCTGATCCTGTACTTCCG
ACGTAATCAGCTTGGCCTGTTCGTCCTGTTGTAGAGTCTCGCCTTTCCGGTCGTTGGCCG
CTTGTTGGCTTAGCTCGGCGATGAGACGATCGTCCCAGACTATACCCGAAGAGCCCTGTC
GACGGAAAGAGATGGTGGATCTCTGAAGACCCGCCATGGACGGCGCTNACTGTTCCTCCT
CCGTTTTTTTCTTCTTCTTTTACTCTGTTTTCGTCGTTTTTGATGTGTGTTGCTAATTCA
CAATGATTGGTAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAA
TGTAGATGTCCGC
   
Sequence Name 70-K103358-0022-902-G09C-8409
GenBank Accession LN512370 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K103358-0022-902-G09C-8409
ATTTACAATTACCAATCATTGTGAATTAGCAACACACATCAAAAACGACTAAAACAGAGT
AAAAGAAGAAGAAAAAAACGGAGGAGGAACAGTTAGCGCCGTCCATGGCGGGTCTTCAGA
GATCCACCATCTCTTTCCGTCGACAGGGCTCTTCGGGTATAGTCTGGGACGATCGTCTCA
TCGCCGAGCTAAGCCAACAAGCGGCCAACGACCGGAAAGGCGAGACTCTACAACAGGACG
AACAGGCCAAGCTGATTACGTCGGAAGTACAGGATCAGACAACCAAACCTATCGCCGGAG
AAGGACTCAAACCTATCAGAACTGACGGAGGTATGGAGAGGAGTCGATCCAACGGAGGAG
GAGCCATCCGCCACCATCGAAACACAGGGAGAGTGTCTCCGGCGGTGGACCCGCCGTCGC
CGAGACTGTCGGCGTTTGGGTGCTGCTCTGCCTTCGGGAAGAAACAGCCGGGGAAGAAGG
TTAATCAGAGGAAAAGGCCAGCGAAGCGTAGATCCAGATAGGCATGAACTAAAGTCTAAA
GTAAAGAAGAAGAACACACACAAAGATGAGAAAACAAAACAAAACAAAAAGAGAGTATAT
ATACTTATACTGCTTCTTATGAATCTTTTTCTTTCTGTTTTTGTTTGTGTTTTTTTGTTT
GTGCTTTAATGTTAAAACTGTTTAAACTCAGAGACTATATATATGTTTATATTTGTTTTG
TTTTTGT
Primers used for PCR 34au - 8474, show primer details
   
Sequence Name 88-K118414-0022-902-G09C-34au
GenBank Accession LN514897 [GenBank]
Sequencing primer gene specific primer, 34au: 5'-ATTCTACTCCCATAATTACCCGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K118414-0022-902-G09C-34au
TTACGCATCTAAACATGATAATCGCGATTATATTTGGTTGGATAATTCGAGAACCAGAAG
AAAAAAAACATATAAAATTGAAATAATAAGACGACAAAAGAAAATTAATTAGAAAAGACA
CAAGTGCCTTCTAATAAAGAGATAAGAAAGAGGAGGAAGAAATGAACTAGTCTGAAACAC
AATGATGGCTTCAGAGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCACGATTATTAT
   
Sequence Name 40-K118413-0022-902-G09C-oriL
GenBank Accession LN503870 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K118413-0022-902-G09C-oriL
TTTACAATTGAATATATCTCTGAAGCCATCATTGTGTTTCAGACTAGTTCATTTCTTCCT
CCTCTTTCTTATCTCTTTATTAGAAGGCACTTGTGTCTTTTCTAATTAATTTTCTTTTGT
CGTCTTATTATTTCAATTTTATATGTTTTTTTTCTTCTGGTTCTCGAATTATCCAACCAA
ATATAATCGCGATTATCATGTTTAGATGCGTAATCATTGTATTTTATTTGTTACGTATTT
TAAACGGGAGAGAAAAAACTGGGTCAAAGCAGCGGGTAATTATGGGAGTAGAAT
Primers used for PCR 75wl - 8474, show primer details
   
Sequence Name 87-K118414-0022-902-G09C-75wl
GenBank Accession LN514803 [GenBank]
Sequencing primer gene specific primer, 75wl: 5'-AGGACTTCCATAATTGTAGCGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K118414-0022-902-G09C-75wl
ATTTATTGGGGATTAATTACGATATGAACACGCAAATTTTGTTGTCCATATGAAAAAAGT
AGCACATGAATGGATTGTCTCCACATTACACAAATGGGATGGTCCAGCAAGCCATATAGC
AAGATATCTTACAAAATAAAATAATCCACACCTCTCAAAGTTGAGCCATTATATTTCAGG
AAATATTAGGTTCGAGTTTTGCGTGATTATTATTTTAAAACTCGATGAATCAGTCATCTA
CTAATACTTGTAAGTTTCAACTTTGAACTAACCAAATCAAGCATGAACAACGACGAGTCA
CCAACGCTTGATTTCCTTAAGAAATATTAAAAAAAATTAAGCCATGCTGGATTATGGTTG
ATAAAACTATATGCTATGCGATGATCTTTGCCTGAAACGTGAAAAGAAAAATAATCATTT
TTGGCAGATAATTAAGTGGCTATAGCTAAATTTAATGTTAAAAAAAAAGATAATTGAAAT
TTCAACACATACCACCCAAAAACGTTGTTTGTACTATTCTACTCCCATAATTACCCGCTG
CTTTGACCCAGTTTTTTCTCTCCCGTTTAAAATACGTAACAAATAAAATACAATGATTAC
GCATCTAAACATGATAATCGCGATTATATTTGGTTGGATAATTCGAGAACCAGAAGAAAA
AAAACATATAAAATTGAAATAATAAGACGACAAAAGAAAATTAATTAGAAAAGACACAAG
TGCCTTCTAATAAAGAGATAAGAAAGAGGAGGAAGAAATGAACTAGTCTGAAACACAATG
ATGGCTTCAGAGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTTC
ATTCAAAATGT
   
Sequence Name 39-K118413-0022-902-G09C-8409
GenBank Accession LN503517 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K118413-0022-902-G09C-8409
TTTACAATTGAAATATCTCTGAAGCCATCATTGTGTTTCAGACTAGTTCATTTCTTCCTC
CTCTTTCTTATCTCTTTATTAGAAGGCACTTGTGTCTTTTCTAATTAATTTTCTTTTGTC
GTCTTATTATTTCAATTTTATATGTTTTTTTTCTTCTGGTTCTCGAATTATCCAACCAAA
TATAATCGCGATTATCATGTTTAGATGCGTAATCATTGTATTTTATTTGTTACGTATTTT
AAACGGGAGAGAAAAAACTGGGTCAAAGCAGCGGGTAATTATGGGAGTAGAATAGTACAA
ACAACGTTTTTGGGTGGTATGTGTTGAAATTTCAATTATCTTTTTTTTTAACATTAAATT
TAGCTATAGCCACTTAATTATCTGCCAAAAATGATTATTTTTCTTTTCACGTTTCAGGCA
AAGATCATCGCATAGCATATAGTTTTATCAACCATAATCCAGCATGGCTTAATTTTTTTT
AATATTTCTTAAGGAAATCAAGCGTTGGTGACTCGTCGTTGTTCATGCTTGATTTGGTTA
GTTCAAAGTTGAAACTTACAAGTATTAGTAGATGACTGATTCATCGAGTTTTAAAATAAT
AATCACGCAAAACTCGAACCTAATATTTCCTGAAATATAATGGCTCAACTTTGAGAGGTG
TGGATTATTTTATTTTGTAAGATATCTTGCTATATGGCTTGCTGGACCATCCCATTTGTG
TAATGTGGAGACAATCCATTCATGTGCTACTTTTTTCATATGGACAACAAAATTTGCGTG
TTCATATCGTAATTAATCCCCAATTAAATCTACATCGCAAATCTAATATACCCATGCATT
TCAAATCTCGCTACA

Last Updated on 06.12.2018 16:11