Sequences from confirmation amplicon


Confirmation sequences for line 918A08 and hit At1g16070 are shown.
Line ID 918A08
Locus At1g16070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5513616
Primers used for PCR BU43 - 8474, show primer details
   
Sequence Name 33-K104354-0022-918-A08C-BU43
GenBank Accession LN500981 [GenBank]
Sequencing primer gene specific primer, BU43: 5'-AAGTCCATCTGTGTCTTTGGTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K104354-0022-918-A08C-BU43
ATTCAATGGAAACCTGTGGGACTTTATAAAAACGTTACTTTCAACTCAGTACCATTGTCA
AACACCTGTTTCTAGATTCATGTCTTCAAAATCTATAAAGATATAAACTTAGCTCCCAGA
AAGTTCTAAACTTGATACTTGAAAGCAATCCATCCAGAAAGGGAAAAAAAACATGGCTGG
TTCGAGAAAAGTGAATGATTTGTTGGAGGAAAATAAGGGAAATGTGGACACAATTACAGG
GTCTTTATCCACTCAAAAGGGAGAGGATAAGGAGAATGTGTCGCCGGAGAAAGTCTCTAC
CTCTGTGGAAACTCGGAAACTAGATCGAGCTTTGAAAAAATCATACAGATATATCAGTCA
CACATGTGTGATCCCCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCACACGGTTATT
   
Sequence Name 36-K104355-0022-918-A08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K104355-0022-918-A08C-8409
CCTTTCATTGGGGATACCATGGGATGAATATCTGTAGATTTTTCACTCATCATTTCCGAG
TTCCCAAGTAAACTTTCCCGGCACCATTCCCTTACCTCCCTTTGAGTGAAAAG

Last Updated on 06.12.2018 16:11