Sequences from confirmation amplicon


Confirmation sequences for line 918D08 and hit At5g38710 are shown.
Line ID 918D08
Locus At5g38710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15501264
Primers used for PCR HM90 - 8474, show primer details
   
Sequence Name 05-K101221w-0022-918-D08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K101221w-0022-918-D08C-8409
AACGCTTGTGAAAATAAAAAGAGAAACCCAAAAACAGAACAAAAGAAAGAAAACAAAACG
TTCTTAAGAGTTTAAATGGCAAACCGTTTCCTCCGACCAAACCTCATCCACCGTTTCTCC
ACCGTGAGTCCCGTCGGTCCTCCGACCACCATCATCCCAGAAATCCTTTCCTTTGACCAA
CCAAAACCAGAAGTTGATCTAGATCTCTCCGACCAAGCTCGACTCTTTGCTTCTGTCCCA
ATCTCCACCCTCCTCCGCTCAACCGCAATTCTCCATGCCACGTCCATAGGCCCTATGGTC
GATCTTGGCTCGTGGCTCATGAGTTCCAAGCTCATGGACACGACCGTTACACGCGATCTA
GTACTCCGTATCGTGAAAGGAACGTTTTACGACCATTTCTGCGCCGGTGAAGACGCGGCT
GCCGCAGCAAGGCGCGTGAGTAGCGTGTATGAGTCGACGGGACTTAAAGGTATGTTA
   
Sequence Name 69-K101228-0022-918-D08C-HM90
GenBank Accession LN512155 [GenBank]
Sequencing primer gene specific primer, HM90: 5'-CTAACATACCTTTAAGTCCCGTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K101228-0022-918-D08C-HM90
GGCGCCGCGTCTTCACCGGCGCAGAAATGGTCGTAAAACGTTCCTTTCACGATACGGAGT
ACTAGATCGCGTGTAACGGTCGTGTCCATGAGCTTGGAACTCATGAGCCACGAGCCAAGA
TCGACCATAGGGCCTATGGACGTGGCATGGAGAATTGCGGTTGAGCGGAGGAGGGTGGAG
ATTGGGACAGAAGCAAAGAGTCGAGCTTGGTCGGAGAGATCTAGATCAACTTCTGGTTTT
GGTTGGTCAAAGGAAAGGATTTCTGGGATGATGGTGGTCGGAGGACCGACGGGACTCACG
GTGGAGAAACGGTGGATGAGGTTTGGTCGGAGGAAACGGTTTGCCATTTAAACTCTTAAG
AACGTTTTGTTTTCTTTCTTTTGTTCTGTTTTTGGGTTTCTCTTTTTATTTTTCACAAGC
GTTTTTGTGTGTGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGACCGCA

Last Updated on 06.12.2018 16:11