Sequences from confirmation amplicon


Confirmation sequences for line 951A08 and hit At4g37490 are shown.
Line ID 951A08
Locus At4g37490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17622524
Primers used for PCR AC97 - 8474, show primer details
   
Sequence Name 51-K104214-0022-951-A08C-AC97
GenBank Accession LN507491 [GenBank]
Sequencing primer gene specific primer, AC97: 5'-TTGTCTTCGGCTAAATGAAAAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K104214-0022-951-A08C-AC97
GGGAGACTGTTGATATTGCAGACCATGCATACAGTCACAAACAGATTCTGGTGATGGAGA
AGACAATACTGTCTACACTTGAGTGGTACTTGACAGTTCCGACTCATTATGTCTTCCTAG
CTCGTTTCATCAAAGCTTCCATTGCAGACGAAAAGGTAAGAGAGCGTTTTTTTAGTTTCT
TCCATGGTTATACATTTCATCATATTGAACATTCAGTTGTGTTTTGGTTTGCAGATGGAG
AATATGGTGCACTATTTGGCTGAGTTAGGCGTAATAGATCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTA
   
Sequence Name 11-K104215-0022-951-A08C-8409
GenBank Accession LN491092 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K104215-0022-951-A08C-8409
CCATTTACAATTGATCTATTACGCCTAACTCAGCCAAATAGTGCACCATATTCTCCATCT
GCAAACCAAAACACAACTGGAATGTTCAATATGATGAAATGTATAACCATGGAAGAAACT
AAAAAAACGCTCTCTTACCTTTTCGTCTGCAATGGAAGCTTTGATGAAACGAGCTAGGAA
GACATAATGAGTCGGAACTGTCAAGTACCACTCAAGTGTAGACAGTATTGTCTTCTCCAT
CACCAGAATCTGTTTGTGACTGTATGCATGGTCTGCAATATCAACTAGATCCTCCACCTG
CAAAAGAATTATCTTTTCATTTACCGGAAGACA

Last Updated on 06.12.2018 16:11