Sequences from confirmation amplicon


Confirmation sequences for line 952G07 and hit At4g20080 are shown.
Line ID 952G07
Locus At4g20080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10867305
Primers used for PCR 32pf - 8474, show primer details
   
Sequence Name 70-K115187-0022-952-G07C-32pf
GenBank Accession LN512439 [GenBank]
Sequencing primer gene specific primer, 32pf: 5'-AGACAATTCCACACTCACAAGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K115187-0022-952-G07C-32pf
GAATTCTGAGGATAGAAGGTATATCGAAAACTCTAACCCTAGGATGTCTCATGACGTAGA
ACGCAAGAAATGTGAGCAGTAACTTCATGGAGACGAAACAAATCACTCCACAGCTAACAA
AACAGAACGTCAAGAACAGAGACGTTGCTCTTGGATCTCTCCAACTCAACAAACTTTTCA
CTCTTTCTCCTTGTGTAGCCAAATCTCCCAACACAATCATCATCCTCCCTGCGATCCCAC
GCAACCTATCATACCTTCTTTTCAGTACATCACCAATGAAAATGGCTTCAATTGTATCAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATA
   
Sequence Name 34-K115186-0022-952-G07C-8409
GenBank Accession LN501530 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K115186-0022-952-G07C-8409
TATATGATACAATTGAAGCCATTTTCATTGGTGATGTACTGAAAAGAAGGTATGATAGGT
TGCGTGGGATCGCAGGGAGGATGATGATTGTGTTGGGAGATTTGGCTACACAAGGAGAAA
GAGTGAAAAGTTTGTTGAGTTGGAGAGATCCAAGAGCAACGTCTCTGTTCTTGACGTTCT
GTTTTGTTAGCTGTGGAGTGATTTGTTTCGTCTCCATGAAGTTACTGCTCACATTTCTTG
CGTTCTACGTCATGAGACATCCTAGGGTTAGAGTTTTCGATATACCTTCTATTCCTCAGA
ATTTCTTCAGGAGATTACCATCAAGAGCTGATAGCATCTTGTGAGTGGGAAATTGTC

Last Updated on 06.12.2018 16:11