Sequences from confirmation amplicon


Confirmation sequences for line SALK_000766 and hit At5g28060 are shown.
Line ID SALK_000766
Locus At5g28060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:10070117
Primers used for PCR 61wg - LBb3, show primer details
   
Sequence Name 64-K118240-SALK_000766C-61wg
GenBank Accession LN485859 [GenBank]
Sequencing primer gene specific primer, 61wg: 5'-TGAACTTCTCATCGTTTTTCTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K118240-SALK_000766C-61wg
GAATATCTAATTCTTATAAAAAAGTTTATATAACTTTTCAATAAACATATTCACTATCTA
GTATTTGTAAATCAATATCTAGTTCTTATAAATCAATATCTAGTATTTTACATGTTAAGT
CTCCCAAATGGGTTCCTTACTTCCTTCTTGTAAAATCCCTAAATTTTTCCAATGGTTTTT
CGGTTTAACATGAGACAAGGAAACATCTATTTTGCTTATTTAGATTGTTGTATCCGTCAT
CGATGTTGCGTTGGTGGAAAATGATAAACCTTTGAATGGTTCATTGATATACATTTGAAA
ATGCAGAAAGTTGAGTTCTTTAGAATATTGCAGTCAAAAATAGAATTCCCCTGTGAAACC
AGAACGTAGTCTTTTTTTCTTTATTCTCATGACAACTAATGAATTTCAAGTAGAATCCAA
CTTTTAATCTTGTGGTATGTGGTAGCAAAAATTGAAAAGTTGAACAATAGTCTCAATCTC
CGAAACGCAAGAGGATGAAGTTTAAAATCAATGTATTCAAACAAAATCTTAACCACATGT
TTCTCTAAAACAAGATCCAAACCAAAATAGAGCAAGTGTGACAAAAAGAAGCACAAAATC
GAGAAATAATTAGAAGATGTCTAATAGCAAACATGGCTTCAAAGAAGGAGTGATAACAAA
AGTTTTTAATTAAAAACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGG
TTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAG
AGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGG
TGGTTC
   
Sequence Name 41-K118239-SALK_000766C-R210
GenBank Accession LN485321 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K118239-SALK_000766C-R210
ACTTTTGTTTCACTCCTTCTTTGAGCCATGTTTGCTATTAGACATCTTCTAATTATTTCT
CGATTTTGTGCTTCTTTTTGTCACACTTGCTCTATTTTGGTTTGGATCTTGTTTTAGAGA
AACATGTGGTTAAGATTTTGTTTGAATACATTGATTTTAAACTTCATCCTCTTGCGTTTC
GGAGATTGAGACTATTGTTCAACTTTTCAATTTTTGCTACCACATACCACAAGATTAAAA
GTTGGATTCTACTTGAAATTCATTAGTTGTCATGAGAATAAAGAAAAAAAGACTACGTTC
TGGTTTCACAGGGGAATTCTATTTTTGACTGCAATATTCTAAAGAACTCAACTTTCTGCA
TTTTCAAATGTATATCAATGAACCATTCAAAGGTTTATCATTTTCCACCAACGCAACATC
GATGACGGATACAACAATCTAAATAAGCAAAATAGATGTTTCCTTGTCTCATGTTAAACC
GAAAAACCATTGGAAAAATTTAGGGATTTTACAAGAAGGAAGTAAGGAACCCATTTGGGA
GACTTAACATGTAAAATACTAGATATTGATTTATAAGAACTAGATATTGATTTACAAATA
CTAGATAGTGAATATGTTTATTGAAAAGTTATATAAACTTTTTTATAAGAATTAGATATT
TCTACCAAAATAATATTAAAAGAATAGAAAAAGTATAAGAGAGGAGAAAAACGATGAGAA
AGTTC
Primers used for PCR 27wy - LBb3, show primer details
   
Sequence Name 27-K118859-SALK_000766C-27wy
GenBank Accession LN485074 [GenBank]
Sequencing primer gene specific primer, 27wy: 5'-GAAAAAGCTGTCACTATCAGGACC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K118859-SALK_000766C-27wy
AGCATTCGTGAGTTCCCATCTCTATGTTCCATTTTGCAATCTCACAGTGTTACGTCTTCG
CTGTATCGAATGATAAAAACTATAGTTTAGGTAGAAACAATCTTATAAGAACAGATTCAT
TGATCATTTTGATGTATTGCTAGAGGAAATTTTGAATTTTGCAACTTTTAGGTTTGATGT
TTAGTATTTGGATTCATTTCGATGTTGCATTTGGAGTATTAATCATCAGATTTTGAATCA
ATTAATGTAGGTTATTGATGTTCTTCATCCTGGAAGAGCCAATGTTTCAAAGGTATGTTC
TTTACGTACATTGGCTTGTGTTGATTACAATCCAAGCTTGTGCAGTTGTGTATTGTTTCT
TAGTGATGTTGTGTACTGGGTGGGGCTTTACTTGTTTGAAGGCTGAGCTTAAGGAGAAAT
TGGCGAGGATGTATGAGGTTAAGGACCCAAATGCTATCTTCTGTTTCAAGTTCAGAACTC
ACTTTGGAGGTGGTAAATCTTCTGGATATGGTTTGATCTATGATACTGTCGAGAACGCTA
AGAAGTTTGAGCCTAAGTACAGACTTATCAGGGTAATTTCTCTTTACTTGTTTGATAGTT
AAGTAAAACTTTTGGTTCTAGTTTTTAAACAAATTGCGGACAACTTAATAACACATTGCG
GACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAT
TGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCA
GCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 03-K118858-SALK_000766C-R210
GenBank Accession LN484355 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K118858-SALK_000766C-R210
AAGTTTTACTTAACTATCAAACAAGTAAAGAGAAATTACCCTGATAAGTCTGTACTTAGG
CTCAAACTTCTTAGCGTTCTCGACAGTATCATAGATCAAACCATATCCAGAAGATTTACC
ACCTCCAAAGTGAGTTCTGAACTTGAAACAGAAGATAGCATTTGGGTCCTTAACCTCATA
CATCCTCGCCAATTTCTCCTTAAGCTCAGCCTTCAAACAAGTAAAGCCCCACCCAGTACA
CAACATCACTAAGAAACAATACACAACTGCACAAGCTTGGATTGTAATCAACACAAGCCA
ATGTACGTAAAGAACATACCTTTGAAACATTGGCTCTTCCAGGATGAAGAACATCAATAA
CCTACATTAATTGATTCAAAATCTGATGATTAATACTCCAAATGCAACATCGAAATGAAT
CCAAATACTAAACATCAAACCTAAAAGTTGCAAAATTCAAAATTTCCTCTAGCAATACAT
CAAAATGATCAATGAATCTGTTCTTATAAGATTGTTTCTACCTAAACTATAGTTTTTATC
ATTCGATACAGCGAAGACGTAACACTGTGAGATTGCAAAATGGAACATAGAGATGGGAAC
TCACGAATTGCTTTCTGGCGAGAAGCCTGTTGGTCATGAAGTTTCTGGTCCTGATAGTGA
GGCCCTTTTT

Last Updated on 06.12.2018 16:11