Sequences from confirmation amplicon


Confirmation sequences for line SALK_000997c and hit At3g50520 are shown.
Line ID SALK_000997c
Locus At3g50520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18748059
Primers used for PCR 25sr - LBb3, show primer details
   
Sequence Name 53-K116476-SALK_000997C-25sr
GenBank Accession LN485587 [GenBank]
Sequencing primer gene specific primer, 25sr: 5'-CTTGTTGTCTTCCTGCATCATTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K116476-SALK_000997C-25sr
CAAATTATAACCACATTTCAACATCTCTAATCAAAATTCCCAATAACTATACATTCACAA
AGATGCTAATCATTACTAAATACAAGATAAAGATTCAAAATTTACGATTTCTAAGTCAAG
TTTCGATTCCAAAGATACAGATACAAACTCCATTATTGTTAAATTTACCTGGATTTTTCT
CTCGGCATTCCAAGATGTTTCACCATGACGAACAACAACAATCTCAGCATAATCAACATC
TTCAGAACTGTAGTAAAACCAACAAAGAGATTTCGTCAAAAACACGATCAAAGATGCAAA
TAACGAGAAGAAGCTAGAAGAAGAAGCATACTCGAATCTTGATTCCTCCATGGACAAATT
GTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGT
ACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCC
TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCC
TGTTTGATGGTGGTTCCAAA
   
Sequence Name 51-K116475-SALK_000997C-R210
GenBank Accession LN485541 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K116475-SALK_000997C-R210
ATTTGTTACCCCAATTTGTCCTGGAGGAATAAGATTCGAGTATGCTTCTTCTTCTAGCTT
CTTCTCGTTATTTGCATCTTTGATCGTGTTTTTGACGAAATCTCTTTGTTGGTTTTACTA
CAGTTCTGAAGATGTTGATTATGCTGAGATTGTTGTTGTTCGTCATGGTGAAACATCTTG
GAATGCCGAGAGAAAAATCCAGGTAAATTTAACAATAATGGAGTTTGTATCTGTATCTTT
GGAATCGAAACTTGACTTAGAAATCGTAAATTTTGAATCTTTATCTTGTATTTAGTAATG
ATTAGCATCTTTGTGAATGTATAGTTATTGGGAATTTTGATTAGAGATGTTGAAATGTGG
TTATAATTTGGTGTTACAGGGTCATTTGGATGTTGAGTTAAATGATGCAGGAGAACAACA
AG

Last Updated on 06.12.2018 16:11