Sequences from confirmation amplicon


Confirmation sequences for line SALK_000997c and hit F2N1 are shown.
Line ID SALK_000997c
Locus F2N1 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:499178
Primers used for PCR 01rb - LBb3, show primer details
   
Sequence Name 62-K116055-SALK_000997C-01rb
GenBank Accession LN485801 [GenBank]
Sequencing primer gene specific primer, 01rb: 5'-CTTTTCAAAAACACCACCCATACT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K116055-SALK_000997C-01rb
TTTTTTTGTCCTTGTNAATTTGGTTTCGAACTATGATTATGTTTTTAGTTGGAAATCAAT
ATCATCAATACAATAAAAACACTAGATTTTTCTCGTGTTGACATATCATTAAAAATTACT
ATAACATTAATACAACTCAGCTCTGCGTAGGTATGAACTATGAAGCTGTGTGTTGTGTTT
CATTTTCCTCTGTAATTTGTAATTAAGTAATCGAAATTTTCATTAAAGAAGATAACGAAT
AACATCATGTTGTAATTCTCACATTCTCTCTATCTCTTTCTCTGTTCTTAGTTTCTTTGT
TCTAGAGAGTATAATATTACTCTGTACTTAGTGAGTCATGGTAGTTGATAGGTATTTAAT
TGCAAGAATATCTCTATACTGGCAAAAACAAGGTGAAATAAAAGTTTGTTTTGCGAATAC
CAAATCCATCAAAAGGTAATGTAACCCAATGCATGCTCAAGTGTGTCTGAGAATAATAAT
TTATATAGCCGTCGTAGCCACTCTCAAGTCTTATCTCACGTCTCTTCATATTCTCCTTTT
ATCTCCAAAATTTCCTTAATGGTGTAGCAAAACTTGATATCTGCATTTAAACAAATTGAC
GCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTT
TCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCA
GCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 35-K116054-SALK_000997C-R210
GenBank Accession LN485222 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K116054-SALK_000997C-R210
AAACAAACATTTATTCCACCTTGTTTTTGCCAGTACCGAGATATCCTTGCAAAAAACCAC
CTATCAACGACCATGACTCACTAAGTACAGAGTAATATTATACTCGCTAGAACGAACAAA
CCAAGAACAGAGAAAGAGATAGTTAGAATGTGAGAATTACAACATGATGTTATACGTTAT
CTGCTTTAACGAAAATGTCGATTACTCACTTACGAATTACAGAGGAAAATGAAACACAAC
ACTCTGCTTCATAGTTCATACCTACCCGCAGCTGAGTTGAATTAATGTAACAGTAATTTT
AAATGATACGCCAACACGACAAAAATCTAGTGTTTTTATTGTATTGATGATACTGATTTC
CAACTAAAAACATAATCATAGTTCGAAACCAAATTACACGAGGACAAAAAAATAGCTTTA
CTCATAGACTGACGTTGAAGTAGGGGTGGTGTTTTAGAAAAGAATGGAAATCCCCC
Primers used for PCR 03ry - LBb3, show primer details
   
Sequence Name 08-K116171w-SALK_000997C-03ry
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 03ry: 5'-AAGCTGTGTGTTGTGTTTCATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K116171w-SALK_000997C-03ry
ATTTTCTTAAGAAGATAACGAATAACATCATGTTGTAATTCTCACATTCTCTCTATCTCT
TTCTCTGTTCTTAGTTTCTTTGTTCTAGAGAGTATAATATTACTCTGTACTTAGTGAGTC
ATGGTAGTTGATAGGTATTTAATTGCAAGAATATCTCTATACTGGCAAAAACAAGGTGAA
ATAAAAGTTTGTTTTGCGAATACCAAATCCATCAAAAGGTAATGTAACCCAATGCATGCT
CAAGTGTGTCTGAGAATAATAATTTATATAGCCGTCGTAGCCACTCTCAAGTCTTATCTC
ACGTCTCTTCATATTCTCCTTTTATCTCCAAAATTTCCTTAATGGTGTAGCAAAACTTGA
TATCTGCATTTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTA
ATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAC
CGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAA
ATCCTGTTTGATGGTGGTTCCGAATGGGGCAAAAT
   
Sequence Name 02-K116170w-SALK_000997C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K116170w-SALK_000997C-R210
AATGCAGATATCAAGTTTTGCTACACCATTAAGGAAATTTTGGAGATAAAAGGAGAATAT
GAAGAGACGTGAGATAAGACTTGAGAGTGGCTACGACGGCTATATAAATTATTATTCTCA
GACACACTTGAGCATGCATTGGGTTACATTACCTTTTGATGGATTTGGTATTCGCAAAAC
AAACTTTTATTTCACCTTGTTTTTGCCAGTATAGAGATATTCTTGCAATTAAATACCTAT
CAACTACCATGACTCACTAAGTACAGAGTAATATTATACTCTCTAGAACAAAGAAACTAA
GAACAGAGAAAGAGATAGAGAGAATGTGAGAATTACAACATGATGTTATTCGTTATCTTC
TTTAATGAAAATTTCGATTACTTAATTACAAATTACAGAGGAAAATGAAACACACATCAC
AGCTTA

Last Updated on 06.12.2018 16:11