Sequences from confirmation amplicon


Confirmation sequences for line SALK_001251 and hit At1g01453 are shown.
Line ID SALK_001251
Locus At1g01453 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:166928
Primers used for PCR 32wu - LBb3, show primer details
   
Sequence Name 89-K119308-SALK_001251C-32wu
GenBank Accession LN486330 [GenBank]
Sequencing primer gene specific primer, 32wu: 5'-AACGAAAGTCAAAGGCTTTATTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K119308-SALK_001251C-32wu
GACGACGGTGAGGAAACGACAGGTGGATGATCCGACGGCAAAGAGGCTACAGGTAGAGCT
GAAGAGTAAGAAGCTGTTGGTGACAGTGACAGCTAAGACGAAGGTAGGATTGGCTGTGGG
CAGTCGTAAGATTGTTACTGTGGGCGTTAGTCTCAGATGCGGAGGCGTTATATTACAGAC
GCTTGACTCAAAAATGGCCCAATGCACCATCAAAATGCTCAAATGGTACGTAGAAACTAC
CTGGTCGAAATTAGATGATTTGGTCGATAGTAAACTAAATAAATCATATTACAAAAAGGT
ATTTATATAAACAAATTGACGCTTAGACAACTTGACGCTTAGACAACTTAATAACACATT
GCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT
GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCC
CCAGCAGGCGAAAATCCTGTTTGATGGTGATTCCGAAATGGGCAAAAT
   
Sequence Name 41-K119307-SALK_001251C-R210
GenBank Accession LN485324 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K119307-SALK_001251C-R210
CTAGCGTCATTTGTTTATATAAATACCTTTTTGTAATATGATTTATTTAGTTTACTATCG
ACCAAATCATCTAATTTCGACCAGGTAGTTTCTACGTACCATTTGAGCATTTTGATGGTG
CATTGGGCCATTTTTGAGTCAAGCGTCTGTAATATAACGCCTCCGCATCTGAGACTAACG
CCCACAGTAACAATCTTACGACTGCCCACAGCCAATCCTACCTTCGTCTTAGCTGTCACT
GTCACCAACAGCTTCTTACTCTTCAGCTCTACCTGTAGCCTCTTTGCCGTCGGATCATCC
ACCTGTCGTTTCCTCACCGTCGTCGGGACAATCACAGCCGTCCGATTCCCTGGCTTCTCA
ATAAAGCCTTTGACTTTCGTT

Last Updated on 06.12.2018 16:11