Sequences from confirmation amplicon


Confirmation sequences for line SALK_001468c and hit At2g46470 are shown.
Line ID SALK_001468c
Locus At2g46470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19072781
Primers used for PCR 21mh - LBb3, show primer details
   
Sequence Name 76-K114279-SALK_001468C-21mh
GenBank Accession LN486156 [GenBank]
Sequencing primer gene specific primer, 21mh: 5'-GGTTTTAAGGCCCATTAAGGTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K114279-SALK_001468C-21mh
TGAACGGTGCGTTTTTTGTTGCGATGGTCGTCGTCAGGTAGAGAGAGACGCGAAGTCCTG
ATCGCTCTCTCAGGTCTTAGCCCTAAGACTACGAGTCGACTCAGCCGAACTGTGAATGCA
GTTTTGATTTCAATCCCTTAGGTAATTTTGAAACTTTCGGTTTCTCACTCTTCTCGTTGT
GCTCTCTCAAGAAAATGTTGTGAAGCTTGAAATGAATCCTTATCTGTACAAGTAAAAGCA
GAAATTGATGATCTTTGAGGAACTTATTAGACTGGTTTTGATTCGAATTTTATCTCTTGT
GTATTTGTTGTTGCTATAAGAACTCTCTTTTTGTTAGCAGGATCTTAGTTATGGCTACTT
GCTTGCGTGGAATCACGAAGAGAGTTAATCTTCTACAGAGACGAGTTTATCCATCGTGTG
GTCATCTAATTCGTGATGATAGAGATGAAACTAAATCTGGTTCTTCGGATACAATGATTC
GTGAAGTCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTT
TTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGA
GTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGG
TTCCG
   
Sequence Name 62-K114278-SALK_001468C-R210
GenBank Accession LN485799 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K114278-SALK_001468C-R210
TGTATCCGAAGAACCAGATTTAGTTTCATCTCTATCATCACGAATTAGATGACCACACGA
TGGATAAACTCGTCTCTGTAGAAGATTAACTCTCTTCGTGATTCCACGCAAGCAAGTAGC
CATAACTAAGATCCTGCTAACAAAAAGAGAGTTCTTATAGCAACAACAAATACACAAGAG
ATAAAATTCGAATCAAAACCAGTCTAATAAGTTCCTCAAAGATCATCAATTTCTGCTTTT
ACTTGTACAGATAAGGATTCATTTCAAGCTTCACAACATTTTCTTGAGAGAGCACAACGA
GAAGAGTGAGAAACCGAAAGTTTCAAAATTACCTAAGGGATTGAAATCAAAACTGCATTC
ACAGTTCGGCTGAGTCGACTCGTAGTCTTAGGGCTAAGACCTGAGAGAGCGATCAGGACT
TCGCGTCTCTCTCTACCTGACGACGACCATCGCAACAAAAAACGCACCGTTTCATCTGAC
AAACGAAAACGAAAGACAAAACGCGGCGTTTCGTCTATCTNACCTTAATGGGCTTTTAAA
A

Last Updated on 06.12.2018 16:11